Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)](https://www.abcam.com/ps/products/41/ab41433/Images/ab41433-259092-anti-phospholipase-c-gamma-1-plc-gamma-1-antibody-m156-western-blot.jpg "Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)")
Key features and details
- Mouse monoclonal [M156] to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156]
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Mouse monoclonal [M156] to Phospholipase C gamma 1/PLC-gamma-1 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 (N terminal).
-
Positive control
- WB: Human A431, Hct116, and Jurkat cells; mouse brain. Flow Cyt (intra): Jurkat cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M156 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
|
| Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
|
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
Post-translational
modificationsThe receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
Cellular localization
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
-
Database links
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
Alternative names
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
Images
-
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Phospholipase C gamma 1/PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41433 observed at 160 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab41433 was shown to recognize Phospholipase C gamma 1/PLC-gamma-1 when Phospholipase C gamma 1/PLC-gamma-1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab41433 diluted to 1/1000 and ab181602 (loading control to GAPDH) diluted to 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)](https://www.abcam.com/ps/products/41/ab41433/Images/ab41433-54630-anti-phospholipase-c-gamma-1-plc-gamma-1-antibody-m156-western-blot.jpg)
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)Western blot analysis of Phospholipase C gamma 1/PLC-gamma-1 immunoprecipitates from human jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). Immunoprecipitation was performed with monoclonal anti-Phospholipase C gamma 1/PLC-gamma-1 (ab41433). The blots were probed with anti- Phospholipase C gamma 1/PLC-gamma-1 (lanes 1 & 2) and anti-Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) (lanes 3-6). The latter antibody was used in the presence of phospho- Phospholipase C gamma 1/PLC-gamma-1 (Tyr-775) peptide (lane 5), or a non-specific phosphotyrosine peptide (lane 6).
-
![Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)](https://www.abcam.com/ps/products/41/ab41433/Images/ab41433-54629-anti-phospholipase-c-gamma-1-plc-gamma-1-antibody-m156-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [M156] (ab41433)Overlay histogram showing Jurkat cells stained with ab41433 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41433, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab41433 has been referenced in 3 publications.
- Guedan S et al. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability. J Clin Invest 130:3087-3097 (2020). PubMed: 32069268
- Mizrachy-Schwartz S et al. Up-regulation of AMP-activated protein kinase in cancer cell lines is mediated through c-Src activation. J Biol Chem 286:15268-77 (2011). WB ; Human . PubMed: 21245141
- Li T et al. SH2D4A regulates cell proliferation via the ERalpha/PLC-gamma/PKC pathway. BMB Rep 42:516-22 (2009). PubMed: 19712589