For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/phospholipase-c-gamma-1plc-gamma-1-phospho-y783-antibody-ep1898y-ab76031.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Lipids / Lipoproteins Lipid Metabolism Hydrolysis
Share by email
RecombinantRabMAb

Recombinant Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
  • Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
  • Dot Blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
  • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
  • Suitable for: WB, ICC/IF, Dot blot
  • Reacts with: Mouse, Human

You may also be interested in

Protein
Product image
Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)
Primary
Product image
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y]
    See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies
  • Description

    Rabbit monoclonal [EP1898Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Dot blotmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat cell lysates treated with pervanadate. ICC/IF: Jurkat cells treated with pervanadate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1898Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Hydrolysis
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Hydrolysis
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
  • Recombinant Protein

    • Recombinant Human Phospholipase C gamma 1/PLC-gamma-1 protein (ab112339)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/100000 - 1/200000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
ICC/IF
1/100.
Dot blot
1/1000.
Notes
WB
1/100000 - 1/200000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
ICC/IF
1/100.
Dot blot
1/1000.
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.

Target

  • Function

    Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 EF-hand domain.
    Contains 2 PH domains.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
  • Post-translational
    modifications

    The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
    Ubiquitinated by CBLB in activated T-cells.
  • Cellular localization

    Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
  • Target information above from: UniProt accession P19174 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5335 Human
    • Entrez Gene: 18803 Mouse
    • Omim: 172420 Human
    • SwissProt: P19174 Human
    • SwissProt: Q62077 Mouse
    • Unigene: 268177 Human
    • Unigene: 44463 Mouse
    • Alternative names

      • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
      • Inositoltrisphosphohydrolase antibody
      • Monophosphatidylinositol phosphodiesterase antibody
      • NCKAP3 antibody
      • Phosphatidylinositol phospholipase C antibody
      • Phosphoinositidase C antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-1 antibody
      • Phospholipase C 148 antibody
      • Phospholipase C gamma 1 antibody
      • Phospholipase C-gamma-1 antibody
      • Phospholipase C-II antibody
      • PLC gamma 1 antibody
      • PLC II antibody
      • PLC-148 antibody
      • PLC-gamma-1 antibody
      • PLC-II antibody
      • PLC1 antibody
      • PLC148 antibody
      • Plcg1 antibody
      • PLCG1_HUMAN antibody
      • PLCgamma1 antibody
      see all

    Images

    • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
      Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
      All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/1000 dilution

      Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
      Lane 2 : Jurkat treated with 50mM pervanadate for 5 minutes whole cell lysate
      Lane 3 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 149 kDa
      Observed band size: 149 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
      Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)

      Immunocytochemistry/Immunofluorescence analysis of Jurkat +/- pervanadate (1mM, 30min) and Jurkat + pervanadate (1mM, 30min) + LP labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) with ab76031 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 (goat anti-rabbit IgG Alexa Fluor® 488) (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at a 1/200 dilution. Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.

    • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
      Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
      All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 2 : NIH/3T3 treated with 50ng/ml PDGF-BB for 30 minutes whole cell lysate
      Lane 3 : NIH/3T3 treated with 50ng/ml PDGF-BB for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 149 kDa
      Observed band size: 149 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot Blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
      Dot Blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)

      Dot blot analysis of Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) phospho peptide (Lane 1) and Phospholipase C gamma 1/PLC-gamma-1 non-phospho peptide (Lane 2) labelling Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) with ab76031 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
      Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031)
      All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EP1898Y] (ab76031) at 1/200000 dilution

      Lane 1 : Jurkat cell lysates, untreated
      Lane 2 : Jurkat cell lysates, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 149 kDa
      Observed band size: 149 kDa

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab76031? Please let us know so that we can cite the reference in this datasheet.

    ab76031 has been referenced in 2 publications.

    • Ma ZG  et al. Toll-like receptor 5 deficiency diminishes doxorubicin-induced acute cardiotoxicity in mice. Theranostics 10:11013-11025 (2020). PubMed: 33042267
    • Guedan S  et al. Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability. J Clin Invest 130:3087-3097 (2020). PubMed: 32069268

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab76031.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.