Anti-Phosphoserine antibody (ab9332)
Key features and details
- Rabbit polyclonal to Phosphoserine
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phosphoserine antibody
See all Phosphoserine primary antibodies -
Description
Rabbit polyclonal to Phosphoserine -
Host species
Rabbit -
Specificity
Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin. KLH-phosphoserine conjugate and phosvitin mixture.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.00
Preservative: 0.1% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (7) |
Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
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| Notes |
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WB
Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding. |
Target
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Relevance
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family. -
Alternative names
- phospho-Ser antibody
- pS antibody
- pSER antibody
Images
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Western blot - Anti-Phosphoserine antibody (ab9332)This image is courtesy of an Abreview submitted by Karin Birkenkamp-DemtroederAll lanes : Anti-Phosphoserine antibody (ab9332) at 3 µg/ml (in PBS for 16 hours)
All lanes : Whole cell lysate of monkey COS7 cells
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution
Blocking Step: 10% Milk for 1 hour at room temperature -
Western blot - Anti-Phosphoserine antibody (ab9332)Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (145)
ab9332 has been referenced in 145 publications.
- Ge P et al. M. tuberculosis PknG manipulates host autophagy flux to promote pathogen intracellular survival. Autophagy 18:576-594 (2022). PubMed: 34092182
- Agarwal Y et al. Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity. Nat Biomed Eng 6:129-143 (2022). PubMed: 35013574
- Zhang B et al. Trehalase is required for sex pheromone biosynthesis in Helicoverpa armigera. Insect Mol Biol 31:334-345 (2022). PubMed: 35084068
- Fang Y et al. Age-related GSK3β overexpression drives podocyte senescence and glomerular aging. J Clin Invest 132:N/A (2022). PubMed: 35166234
- Zhang R et al. D-mannose facilitates immunotherapy and radiotherapy of triple-negative breast cancer via degradation of PD-L1. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35181605