Anti-PI-15 antibody (ab133172)
Key features and details
- Goat polyclonal to PI-15
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PI-15 antibody -
Description
Goat polyclonal to PI-15 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human PI-15 aa 128-141 (internal sequence) (Cysteine residue).
Sequence:C-RTGRYRSILQLVKP
Database link: NP_056970.1 -
Positive control
- HeLa cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa).
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Serine protease inhibitor which displays weak inhibitory activity against trypsin. -
Tissue specificity
Weakly expressed. Expressed at low level in prostate, mammary gland, salivary gland and thyroid gland. -
Sequence similarities
Belongs to the CRISP family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 374241 Chicken
- Entrez Gene: 532863 Cow
- Entrez Gene: 51050 Human
- Entrez Gene: 94227 Mouse
- Entrez Gene: 301489 Rat
- Omim: 607076 Human
- SwissProt: Q98ST6 Chicken
- SwissProt: O43692 Human
see all -
Alternative names
- 25 kDa trypsin inhibitor antibody
- CRISP-8 antibody
- CRISP8 antibody
see all
Images
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Western blot - Anti-PI-15 antibody (ab133172)Anti-PI-15 antibody (ab133172) at 0.3 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab133172 has not yet been referenced specifically in any publications.
