Anti-PI4KA antibody (ab111565)
Key features and details
- Rabbit polyclonal to PI4KA
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PI4KA antibody -
Description
Rabbit polyclonal to PI4KA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
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Immunogen
Recombinant fragment corresponding to Human PI4KA aa 1-331.
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Positive control
- 293T, A431, Jurkat and Raji whole cell lysate; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 231 kDa.
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| ICC/IF |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 231 kDa. |
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ICC/IF
1/100 - 1/500. |
Target
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Function
Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. -
Tissue specificity
Expressed ubiquitously. Highest levels in placenta and brain. Little or no expression in lung, liver, pancreas, testis or leukocytes. -
Sequence similarities
Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
Contains 1 PI3K/PI4K domain. - Information by UniProt
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Database links
- Entrez Gene: 282309 Cow
- Entrez Gene: 5297 Human
- Entrez Gene: 224020 Mouse
- Entrez Gene: 64161 Rat
- Entrez Gene: 46720 Xenopus laevis
- Omim: 600286 Human
- SwissProt: P42356 Human
- Unigene: 529438 Human
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Alternative names
- EC 2.7.1.67 antibody
- Phosphatidylinositol 4 kinase alpha antibody
- Phosphatidylinositol 4 kinase catalytic alpha polypeptide antibody
see all
Images
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Western blot - Anti-PI4KA antibody (ab111565)Anti-PI4KA antibody (ab111565) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 231 kDa
5% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-PI4KA antibody (ab111565)ab111565 at 1/200 dilution staining PI4KA in methanol-fixed A431 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab111565 has been referenced in 2 publications.
- Zhang Y et al. Human CPTP promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cells. Int J Biol Sci 18:4963-4983 (2022). PubMed: 35982909
- Azizi F Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P2) Is Required for Rapid Endocytosis in Chromaffin Cells. Biomed Res Int 2020:9692503 (2020). PubMed: 32964048