Anti-Pig Albumin antibody (ab79960)

Thank you for your reply. As I mentioned before normally the discount code has to be issued before purchasing the antibody, in this case I have made an exception. To redeem the discount code below, you must first submit an Abreview on our website (please place the discount code in the notes sections of the Abreview). Also please include as much details as you can in the Abreview. Once the Abreview has been submitted, the discount code will become active and then you may select one antibody free of charge. If there is anything else I can help you with, please let me know. DISCOUNT CODE: ***** Expiration date: ***** I am very pleased to hear you would like to accept our offer and test ab79960 in ICC/IF in pig tissue. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHCn pig tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Thank you for contacting Abcam. The anti-Pig Albumin antibody has not previously been tested in ICC/IF and so we have no standard protocol for this particular antibody, but I have looked over the protocol you sent and have a few questions/tips that may help. 1 - Have you tried staining a pure culture of porcine periodontal ligament epithelial cells and not just a mixed culture? I ask in case there is a possibility that your cells of interest are not surviving in the culture. 2 - Incubating the primary and secondary antibody in PBS rather than the blocking solution, can help increase staining. 3 - You are fixing for 20 minutes in 4% PFA, have you tried fixing for less time, maybe 10-12 minutes. Over fixation of a sample can prevent the antibody from binding to its target. 4 - Using a different blocking solution, either 10% BSA or horse serum etc, could help. Hopefully some of the above suggestions will help resolve the no staining problem. If there is anything  else I can do, please do not hesitate to contact me.