Anti-Pioneer neuron marker antibody [ZNS-2] (ab183372)
![Immunohistochemistry (Frozen sections) - Anti-Pioneer neuron marker antibody [ZNS-2] (ab183372)](https://www.abcam.com/ps/products/183/ab183372/Images/ab183372-224054-anti-pioneer-neuron-marker-antibody-zns-2-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Pioneer neuron marker antibody [ZNS-2] (ab183372)")
Key features and details
- Mouse monoclonal [ZNS-2] to Pioneer neuron marker
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
-
Product name
Anti-Pioneer neuron marker antibody [ZNS-2] -
Description
Mouse monoclonal [ZNS-2] to Pioneer neuron marker -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Zebrafish
Does not react with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
-
Positive control
- Brain, Gut, and Eye tissue.
-
General notes
It is thought that axons of pioneer neurons serve as a pathway for additional neurons that develop later in the embryo and project their axons to the appropriate target. In many systems pioneer neurons are eliminated by programmed cell-death and are not present in the adult.
Location : Fin hair cell, hindbrain horizontal cell, retinal cone cell, retinal inner plexiform layer and pioneer neuron.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
SDS-PAGE -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. -
Clonality
Monoclonal -
Clone number
ZNS-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
Images
-
Immunohistochemistry (Frozen sections) - Anti-Pioneer neuron marker antibody [ZNS-2] (ab183372)This image is courtesy of an Abreview submitted by Dr Ryan MacDonald
ab183372 staining in 4 dpf zebrafish embryo retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and permeabilization was performed using 0.5% Triton X. Blocking of the sample was done with 5% BSA in PBS containing 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab183372 at 1/100 in blocking solution for 16h at 4°C. An alexa 647conjugated goat anti-mouse polyclonal antibody at 1/500 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The IPL and OPL can be observed.
Datasheets and documents
-
Datasheet download
References (0)
ab183372 has not yet been referenced specifically in any publications.
