Recombinant Anti-PKC beta 2 antibody [Y125] (ab32026)
![Flow Cytometry (Intracellular) - Anti-PKC beta 2 antibody [Y125] (ab32026)](https://www.abcam.com/ps/products/32/ab32026/Images/ab32026-505733-anti-pkc-beta-2-antibody-y125-flowcytometry-k562-human.jpg "Flow Cytometry (Intracellular) - Anti-PKC beta 2 antibody [Y125] (ab32026)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y125] to PKC beta 2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-PKC beta 2 antibody [Y125]
See all PKC beta 2 primary antibodies -
Description
Rabbit monoclonal [Y125] to PKC beta 2 -
Host species
Rabbit -
Specificity
ab32026 recognises PKC beta 2, it does not cross react with human PKC beta 1. Immunogen's homology with PKC alpha is 77.8%. Cross-reactivity with PKC alpha has not been tested. -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide within Human PKC beta 2 aa 600-700 (C terminal). The exact sequence is proprietary.
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Epitope
ab32026 reacts with an epitope located in the region near the C terminus of PKC beta 2. -
Positive control
- WB: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate unboiled, Mouse brain lysate unboiled, Rat brain lysate unboiled, Active GST-tagged human PKC beta 2 full length protein. IHC-P: human gastric carcinoma, mouse cerebrum, rat cerebrum. ICC/IF: HeLa (Human cervix adenocarcinoma epithelial cell) Flow cyto(intra): K-562 (Human chronic myelogenous leukemia lymphoblast) cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y125 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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| IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/250 - 1/500.
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| Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/250 - 1/500. |
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. -
Cellular localization
Cytoplasm. Nucleus. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5579 Human
- Entrez Gene: 18751 Mouse
- Entrez Gene: 25023 Rat
- Omim: 176970 Human
- SwissProt: P05126 Cow
- SwissProt: P05771 Human
- SwissProt: P68404 Mouse
- SwissProt: P68403 Rat
see all -
Alternative names
- KPCB_HUMAN antibody
- PKC Beta antibody
- PKC-B antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-PKC beta 2 antibody [Y125] (ab32026)
Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling PKC beta 2 with purified ab32026 at 1/20 dilution (5 ug/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 2 antibody [Y125] (ab32026)](https://www.abcam.com/ps/products/32/ab32026/Images/ab32026-505732-anti-pkc-beta-2-antibody-y125-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 2 antibody [Y125] (ab32026)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PKC beta 2 using ab32026. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab32026 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)](https://www.abcam.com/ps/products/32/ab32026/Images/ab32026-505731-anti-pkc-beta-2-antibody-y125-immunohistochemistry-cerebrum-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)Immunohistochemical analysis of Paraffin-embedded sections rat cerebrum tissue labelling PKC beta 2 with ab32026 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat cerebrum tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)](https://www.abcam.com/ps/products/32/ab32026/Images/ab32026-505730-anti-pkc-beta-2-antibody-y125-immunohistochemistry-cerebrum-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)Immunohistochemical analysis of Paraffin-embedded sections mouse cerebrum tissue labelling PKC beta 2 with ab32026 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse cerebrum tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)](https://www.abcam.com/ps/products/32/ab32026/Images/ab32026-505729-anti-pkc-beta-2-antibody-y125-immunohistochemistry-gastric-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)Immunohistochemical analysis of Paraffin-embedded sections human gastric carcinoma tissue labelling PKC beta 2 with ab32026 at 1/300 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human gastric carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)](https://www.abcam.com/ps/products/32/ab32026/Images/ab32026-505728-anti-pkc-beta-2-antibody-y125-westernblot-recombinant-protein-human.jpg)
Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution
Lane 1 :Recombinant human PKC beta 2 protein (ab60841)
Lane 2 :Recombinant human PKC beta 1 protein (ab60840)
Lane 3 :Recombinant human PKC delta protein (ab60844)
Lane 4 :Recombinant human PKC eta protein (ab60849)
Lane 5 :Recombinant human PKC epsilon protein (ab60847)
Lane 6 :Recombinant human PKC theta/PRKCQ protein (ab56641)
Lane 7 :Recombinant human PKC mu/PKD protein (ab60873)
Lane 8 :Recombinant human PKC alpha protein (Active) (ab55672)
Lane 9 :Recombinant human PKC gamma protein (ab60842)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab235384 was used to detect GST tag.
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![Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)](https://www.abcam.com/ps/products/32/ab32026/Images/ab32026-505727-anti-pkc-beta-2-antibody-y125-westernblot-human-mouse-rat.jpg)
Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate boiled
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate unboiled
Lane 3 : Mouse brain lysate boiled
Lane 4 : Mouse brain lysate unboiled
Lane 5 : Rat brain lysate boiled
Lane 6 : Rat brain lysate unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
We recommend not to boil samples when loading lysate.
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![Anti-PKC beta 2 antibody [Y125] (ab32026)](https://www.abcam.com/ps/products/32/ab32026/Images/ab32026-9-benefits-of-recombinant-antibodies.png)
Anti-PKC beta 2 antibody [Y125] (ab32026)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab32026 has been referenced in 24 publications.
- Zhao L et al. Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ1 signaling pathway in diabetic atherosclerosis. Mol Med 28:4 (2022). PubMed: 35062863
- Kim JM et al. Downregulation of matriptase suppresses the PAR‑2/PLCγ2/PKC‑mediated invasion and migration abilities of MCF‑7 breast cancer cells. Oncol Rep 46:N/A (2021). PubMed: 34608498
- Child SJ et al. Rapid adaptation to human protein kinase R by a unique genomic rearrangement in rhesus cytomegalovirus. PLoS Pathog 17:e1009088 (2021). PubMed: 33497413
- Kim JM et al. Bruton's agammaglobulinemia tyrosine kinase (Btk) regulates TPA-induced breast cancer cell invasion via PLC?2/PKCß/NF-?B/AP-1-dependent matrix metalloproteinase-9 activation. Oncol Rep 45:N/A (2021). PubMed: 33760219
- Pohjolainen L et al. Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Front Pharmacol 11:553852 (2020). PubMed: 33584253