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    products/primary-antibodies/pkc-delta-antibody-epr17075-ab182126.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PKC delta antibody [EPR17075] (ab182126)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (7) Submit a question References (37)

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Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Flow Cytometry (Intracellular) - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
  • Anti-PKC delta antibody [EPR17075] (ab182126)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17075] to PKC delta
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free PE

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PKC Kinase Activity Assay Kit (ab139437)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human PRKCD (PKC delta) knockout HeLa cell line (ab265721)

View more associated products

Overview

  • Product name

    Anti-PKC delta antibody [EPR17075]
    See all PKC delta primary antibodies
  • Description

    Rabbit monoclonal [EPR17075] to PKC delta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-239T, A431, C6, NIH/3T3, HAP1 and HeLa whole cell lysates, human fetal brain and fetal heart, mouse and rat thymus and brain and rat spleen tissue lysates. IHC-P: Human spleen, human transitional cell carcinoma of bladder, mouse liver and rat testis tissues. ICC/IF: Wild-type HAP1 (PMA-treated and untreated) and HeLa cells. Flow Cyt (intra): HeLa cells.
  • General notes

    Protein kinase C delta type (PKCD) is an enzyme encoded for by the PRKCD gene and is expressed ubiquitously among cells and tissues. It is thought that PKCD, when activated in distinct ways, plays critical roles in cellular functions such as the control of growth, differentiation and apoptosis.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17075
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Neuroscience
    • Neurology process
    • Neuroendocrinology
    • Prolactin
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-PKC delta antibody [EPR17075] (ab206282)
    • Alexa Fluor® 647 Anti-PKC delta antibody [EPR17075] (ab206283)
    • Anti-PKC delta antibody [EPR17075] - BSA and Azide free (ab222229)
    • APC Anti-PKC delta antibody [EPR17075] (ab225292)
    • PE Anti-PKC delta antibody [EPR17075] (ab225293)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PRKCD (PKC delta) knockout HeLa cell line (ab265721)
    • Human PRKCD (PKC delta) knockout HEK-293T cell line (ab266143)
  • KO cell lysates

    • Human PRKCD (PKC delta) knockout HEK-293T cell lysate (ab257042)
    • Human PRKCD (PKC delta) knockout HeLa cell lysate (ab257043)
  • Recombinant Protein

    • Recombinant human PKC delta protein (ab60844)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (4)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB (1)
1/5000. Detects a band of approximately 40, 78 kDa (predicted molecular weight: 78 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Treatment with 10nM PMA for 10 min induces translocation of PKCδ to the membrane.

Notes
Flow Cyt (Intra)
1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 40, 78 kDa (predicted molecular weight: 78 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Treatment with 10nM PMA for 10 min induces translocation of PKCδ to the membrane.

Target

  • Function

    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
    The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
  • Post-translational
    modifications

    Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
  • Cellular localization

    Cytoplasm. Membrane.
  • Target information above from: UniProt accession Q05655 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5580 Human
    • Entrez Gene: 18753 Mouse
    • Entrez Gene: 170538 Rat
    • Omim: 176977 Human
    • SwissProt: Q05655 Human
    • SwissProt: P28867 Mouse
    • SwissProt: P09215 Rat
    • Unigene: 155342 Human
    • Unigene: 2314 Mouse
    • Unigene: 98279 Rat
    see all
  • Alternative names

    • CVID9 antibody
    • D14Ertd420e antibody
    • Kinase PKC delta antibody
    • KPCD antibody
    • KPCD_HUMAN antibody
    • MAY 1 antibody
    • MAY1 antibody
    • MGC49908 antibody
    • nPKC delta antibody
    • nPKC-delta antibody
    • PCKd antibody
    • PKC d antibody
    • PKC delta antibody
    • PKCD antibody
    • PKCdelta antibody
    • PRKC D antibody
    • PRKC delta antibody
    • Prkcd antibody
    • Protein Kinase C delta antibody
    • Protein kinase C delta type antibody
    • Protein kinase C delta VIII antibody
    • Protein Kinase Cdelta antibody
    • Tyrosine protein kinase PRKCD antibody
    see all

Images

  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    All lanes : Anti-PKC delta antibody [EPR17075] (ab182126) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PRKCD knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 78 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab182126 observed at 80 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab182126 was shown to react with PKC delta in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265721 (knockout cell lysate ab257043) was used. Wild-type HeLa and PKC delta knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab182126 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)
    Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)

    ab182126 staining PKCδ in untreated wild-type HAP1 cells (top panel) and PKCδ untreated knockout HAP1 cells (bottom panel). Untreated cells show PKCδ being expressed in the cytoplasm. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab182126 at 1/200 dilution and ab7291 at 1ug/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (ab150117) at 2ug/ml (shown in pseudo-color red). Nuclear DNA was labelled in blue with DAPI.

    This product also gave a positive signal under the same testing conditions in HAP1 cells fixed with 100% methanol (5 min).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling PKC delta with ab182126 at 1/2000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic and weak nuclear staining on cells in the seminiferous tubule of rat testis is detected. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    All lanes : Anti-PKC delta antibody [EPR17075] (ab182126) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : PKC delta knockout HAP1 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 78 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab182126 observed at 78 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab182126 was shown to specifically react with PKC delta when PKC delta knockout samples were used. Wild-type and PKC delta knockout samples were subjected to SDS-PAGE. ab182126 and ab8226 (loading control to beta Actin) were diluted to 1/5000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    All lanes : Anti-PKC delta antibody [EPR17075] (ab182126) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PRKCD CRISPR/Cas9 edited HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 78 kDa
    Observed band size: 78 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab182126 observed at 78 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab182126 was shown to react with PKC in wild-type HEK-293T cells in western blot. The bands observed in PRKCD CRISPR/Cas9 edited cell line ab266143 (PRKCD CRISPR/Cas9 edited cell lysate ab257042) below 78kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and PRKCD CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab182126 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4

    176;® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-PKC delta antibody [EPR17075] (ab182126)
    Flow Cytometry (Intracellular) - Anti-PKC delta antibody [EPR17075] (ab182126)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling PKC delta with purified ab182126 at 1/250 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cellswithout incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)
    Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)

    ab182126 staining PKCδ in 10nM PMA-treated wild-type HAP1 cells (top panel) and PKCδ in 10nM PMA-treated knockout HAP1 cells (bottom panel). The cells were treated with 10nM PMA for 10 minutes to induce translocation of PKCδ to the cell membrane. The cells were then fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab182126 at 1/200 dilution and ab7291 at 1ug/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (ab150117) at 2ug/ml (shown in pseudo-color red). Nuclear DNA was labelled in blue with DAPI.

    This product also gave a positive signal under the same testing conditions in HAP1 cells fixed with 100% methanol (5 min).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    All lanes : Anti-PKC delta antibody [EPR17075] (ab182126) at 1/5000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates
    Lane 3 : Rat spleen lysates
    Lane 4 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78 kDa



    The 40kDa band represents the cleaved kinase domain.

     

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PKC delta with ab182126 at 1/2000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on kupffer cells of Mouse liver is detected. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    All lanes : Anti-PKC delta antibody [EPR17075] (ab182126) at 1/5000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal heart lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 78 kDa



    The 40kDa band represents the cleaved kinase domain.

     

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PKC delta with ab182126 at 1/2000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic and weak nuclear staining on cancer cells of bladder transitional cell carcinoma is detected. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)
    Immunocytochemistry/ Immunofluorescence - Anti-PKC delta antibody [EPR17075] (ab182126)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PKC delta with ab182126 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IAlexa Fluor® 488 (IgG) (ab150077) at 1/400 dilution was used as the secondary antibody (green). The confocal image shows both cytoplasmic and nuclear staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (goat anti-mouse AlexaFluor® 594 secondary antibody) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab182126 at 1/100 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    All lanes : Anti-PKC delta antibody [EPR17075] (ab182126) at 1/10000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates at 10 µg
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78 kDa



    The 40kDa band represents the cleaved kinase domain.

     

    Blocking/dilution buffer: 5% NFDM/TBST.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta antibody [EPR17075] (ab182126)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling PKC delta with ab182126 at 1/2000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic and nucleus staining on lymphocytes of Human spleen is detected. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    Western blot - Anti-PKC delta antibody [EPR17075] (ab182126)
    All lanes : Anti-PKC delta antibody [EPR17075] (ab182126) at 1/100000 dilution

    Lane 1 : Mouse thymus lysates
    Lane 2 : Rat thymus lysates

    Lysates/proteins at 10 mg/ml per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 78 kDa



    The 40kDa band represents the cleaved kinase domain.

     

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-PKC delta antibody [EPR17075] (ab182126)
    Anti-PKC delta antibody [EPR17075] (ab182126)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (37)

Publishing research using ab182126? Please let us know so that we can cite the reference in this datasheet.

ab182126 has been referenced in 37 publications.

  • Qi B  et al. Brazilin prevents against myocardial ischemia-reperfusion injury through the modulation of Nrf2 via the PKC signaling pathway. Ann Transl Med 9:312 (2021). PubMed: 33708939
  • Feng L  et al. Hepatic Knockdown of Endothelin Type A Receptor (ETAR) Ameliorates Hepatic Insulin Resistance and Hyperglycemia Through Suppressing p66Shc-Mediated Mitochondrial Fragmentation in High-Fat Diet-Fed Mice. Diabetes Metab Syndr Obes 14:963-981 (2021). PubMed: 33688230
  • Kim JM  et al. Bruton's agammaglobulinemia tyrosine kinase (Btk) regulates TPA-induced breast cancer cell invasion via PLC?2/PKCß/NF-?B/AP-1-dependent matrix metalloproteinase-9 activation. Oncol Rep 45:N/A (2021). PubMed: 33760219
  • Zhu J  et al. Protein Kinase C-d Mediates Kidney Tubular Injury in Cold Storage-Associated Kidney Transplantation. J Am Soc Nephrol 31:1050-1065 (2020). PubMed: 32291286
  • Joseph LC  et al. PKCd causes sepsis-induced cardiomyopathy by inducing mitochondrial dysfunction. Am J Physiol Heart Circ Physiol 318:H778-H786 (2020). PubMed: 32142354
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1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC delta antibody [EPR17075]

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 19 2020

Imaging Mass Cytometry™ abreview for Anti-PKC delta antibody [EPR17075]

Good
Abreviews
Abreviews
abreview image
Application
Mass Cytometry
Sample
Human Tissue sections (Liver)
Specification
Liver
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 20 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC delta antibody [EPR17075]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (palatine tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
palatine tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 24 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC delta antibody [EPR17075]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Hannes Kaddatz

Verified customer

Submitted Jul 24 2020

Western blot abreview for Anti-PKC delta antibody [EPR17075]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - whole (whole body)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Treatment
Fluvastatin different concentrations
Specification
whole body
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Mohamed Al-Sabri

Verified customer

Submitted Dec 09 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC delta antibody [EPR17075]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9
Permeabilization
No
Specification
brain tissue
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Markus Kipp

Verified customer

Submitted Aug 22 2018

Immunohistochemistry (Frozen sections) abreview for Anti-PKC delta antibody [EPR17075]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.1% triton-x100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 27 2015

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