Recombinant Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
![Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)](https://www.abcam.com/ps/products/76/ab76181/Images/ab76181-271436-anti-pkc-delta-phospho-y311-antibody-epr2609y-western-blot.jpg "Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311)
- Suitable for: Dot blot, WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PKC delta (phospho Y311) antibody [EPR2609Y]
See all PKC delta primary antibodies -
Description
Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate treated with Phorbol-12-myristate-13-acetate and U937 cell lysate treated with TPA. IHC-P: Human urinary bladder carcinoma tissue. IP: HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2609Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
|
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| WB | (2) |
1/2500 - 1/5000. Predicted molecular weight: 77 kDa.
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| IP |
1/40.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Dot blot
1/1000. |
|
WB
1/2500 - 1/5000. Predicted molecular weight: 77 kDa. |
|
IP
1/40. |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner. -
Post-translational
modificationsPhosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5580 Human
- Entrez Gene: 18753 Mouse
- Omim: 176977 Human
- SwissProt: Q05655 Human
- SwissProt: P28867 Mouse
- Unigene: 155342 Human
- Unigene: 2314 Mouse
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Alternative names
- CVID9 antibody
- D14Ertd420e antibody
- Kinase PKC delta antibody
see all
Images
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Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/500 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)](https://www.abcam.com/ps/products/76/ab76181/Images/ab76181-269883-anti-pkc-delta-phospho-y311-antibody-epr2609y-dot-blot.jpg)
Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)Dot blot analysis of PKC delta (pY311) phospho peptide (Lane 1) and PKC delta non-phospho peptide (Lane 2) using ab76181 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)](https://www.abcam.com/ps/products/76/ab76181/Images/ab76181-91538-anti-pkc-delta-phospho-y311-antibody-epr2609y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma with ab76181 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)](https://www.abcam.com/ps/products/76/ab76181/Images/ab76181-272982-anti-pkc-delta-phospho-y311-antibody-epr2609y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)ab76181 at 1/20 immunoprecipitating PKC delta (phospho Y311) in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
Lane 1 (input): HeLa treated with 10mM H2O2 for 1 hour whole cell lysate (10µg)
Lane 2 (+): ab76181 + HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76181 in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.For western blotting, ab76181 was used as a dilution of 1/200 followed by ab131366 VeriBlot for IP (HRP) at a dilution of 1/1000.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)](https://www.abcam.com/ps/products/76/ab76181/Images/ab76181-91537-anti-pkc-delta-phospho-y311-antibody-epr2609y-western-blot.jpg)
Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/5000 dilution
Lane 1 : U937 cell lysate
Lane 2 : U937 cell lysate treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit-HRP at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted? -
![Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)](https://www.abcam.com/ps/products/76/ab76181/Images/ab76181-7-benefits-of-recombinant-antibodies.png)
Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab76181 has been referenced in 5 publications.
- Nelson LJ et al. Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C d Pathway in a Subset of Triple-Negative Breast Cancers. Am J Pathol 190:484-502 (2020). PubMed: 31843498
- Shou X et al. Baicalin Suppresses Hypoxia-Reoxygenation-Induced Arterial Endothelial Cell Apoptosis via Suppressing PKCd/p53 Signaling. Med Sci Monit 23:6057-6063 (2017). PubMed: 29272263
- He XJ et al. Roles of 1,25(OH)2D3 and Vitamin D Receptor in the Pathogenesis of Rheumatoid Arthritis and Systemic Lupus Erythematosus by Regulating the Activation of CD4+ T Cells and the PKCd/ERK Signaling Pathway. Cell Physiol Biochem 40:743-756 (2016). PubMed: 27915349
- Gruosso T et al. Chronic oxidative stress promotes H2AX protein degradation and enhances chemosensitivity in breast cancer patients. EMBO Mol Med 8:527-49 (2016). WB . PubMed: 27006338
- Ficarro SB et al. Online nanoflow multidimensional fractionation for high efficiency phosphopeptide analysis. Mol Cell Proteomics 10:O111.011064 (2011). WB ; Human . PubMed: 21788404