Recombinant Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)
![Western blot - Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)](https://www.abcam.com/ps/products/110/ab110728/Images/ab110728-124020-anti-pkc-theta-antibody-epr14872-western-blot.jpg "Western blot - Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1487(2)] to PKC theta/PRKCQ
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PKC theta/PRKCQ antibody [EPR1487(2)]
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit monoclonal [EPR1487(2)] to PKC theta/PRKCQ -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human platelet and Jurkat lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1487(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 82 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 82 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Tissue specificity
Skeletal muscle, megakaryoblastic cells and platelets. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Domain
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
Post-translational
modificationsAutophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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Database links
- Entrez Gene: 5588 Human
- Entrez Gene: 18761 Mouse
- Entrez Gene: 85420 Rat
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q02111 Mouse
- SwissProt: Q9WTQ0 Rat
- Unigene: 498570 Human
see all -
Alternative names
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
Images
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Western blot - Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)All lanes : Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728) at 1/1000 dilution
Lane 1 : Human platelet lysate
Lane 2 : Jurkat lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 82 kDa -
![Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)](https://www.abcam.com/ps/products/110/ab110728/Images/ab110728-2-benefits-of-recombinant-antibodies.png)
Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab110728 has been referenced in 6 publications.
- Chi JN et al. MAP4K3/GLK inhibits Treg differentiation by direct phosphorylating IKKβ and inducing IKKβ-mediated FoxO1 nuclear export and Foxp3 downregulation. Theranostics 12:5744-5760 (2022). PubMed: 35966593
- van Senten JR et al. Use of CRISPR/Cas9-edited HEK293 cells reveals that both conventional and novel protein kinase C isozymes are involved in mGlu5a receptor internalization. J Biol Chem 298:102466 (2022). PubMed: 36087841
- Søgaard D et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). PubMed: 30871020
- Byerly J et al. PRKCQ promotes oncogenic growth and anoikis resistance of a subset of triple-negative breast cancer cells. Breast Cancer Res 18:95 (2016). PubMed: 27663795
- Bracamontes CG et al. The serum protein profile of early parity which induces protection against breast cancer. Oncotarget 7:82538-82553 (2016). WB ; Human . PubMed: 27769065
- Søgaard D et al. Training Does Not Alter Muscle Ceramide and Diacylglycerol in Offsprings of Type 2 Diabetic Patients Despite Improved Insulin Sensitivity. J Diabetes Res 2016:2372741 (2016). PubMed: 27777958