Anti-Placental lactogen antibody (ab15554)
Key features and details
- Rabbit polyclonal to Placental lactogen
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Placental lactogen antibody
See all Placental lactogen primary antibodies -
Description
Rabbit polyclonal to Placental lactogen -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Placental lactogen.
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Positive control
- Placenta
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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| IHC-P |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
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IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Function: Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization. Similarity: Belongs to the somatotropin/prolactin family. -
Database links
- Entrez Gene: 1442 Human
- Entrez Gene: 1443 Human
- Omim: 118820 Human
- SwissProt: P01243 Human
- SwissProt: P0DML3 Human
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Alternative names
- Choriomammotropin antibody
- Chorionic somatomammotropin A antibody
- chorionic somatomammotropin B antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental lactogen antibody (ab15554)
ab15554 staining Human placenta by Immunohistochemistry (FFPE-sections).
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Western blot - Anti-Placental lactogen antibody (ab15554)This image is courtesy of an Abreview submitted by Dr Stoil DmitrovAnti-Placental lactogen antibody (ab15554) at 1/100 dilution + Human recombinant hPL at 0.1 µg
Secondary
HRP conjugated donkey anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 10 kDa, 14 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
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Western blot - Anti-Placental lactogen antibody (ab15554)Anti-Placental lactogen antibody (ab15554) at 1/25 dilution + Human placenta lysate
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab15554 has been referenced in 7 publications.
- Drwal E et al. "Real life" polycyclic aromatic hydrocarbon (PAH) mixtures modulate hCG, hPL and hPLGF levels and disrupt the physiological ratio of MMP-2 to MMP-9 and VEGF expression in human placenta cell lines. Reprod Toxicol 95:1-10 (2020). PubMed: 32418891
- Martinez ME et al. Cotton Rat Placenta Anatomy and Fc Receptor Expression and Their Roles in Maternal Antibody Transfer. Comp Med 70:510-519 (2020). PubMed: 33121562
- Dawid M et al. Apelin decreased placental hormone secretion by human trophoblast BeWo cells via apelin receptor, protein kinase A and extracellular signal-regulated kinases 1/2 activation. J Physiol Pharmacol 70:N/A (2019). PubMed: 32084650
- La Perle KM et al. Trophoblast deportation to the lungs of cotton rats (Sigmodon hispidus). Comp Med 64:448-55 (2014). PubMed: 25527025
- Drukker M et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). ICC/IF ; Human . PubMed: 22634564
- Adachi K et al. Role of SOX2 in maintaining pluripotency of human embryonic stem cells. Genes Cells : (2010). PubMed: 20384793
- McDonald EA & Wolfe MW Adiponectin attenuation of endocrine function within human term trophoblast cells. Endocrinology 150:4358-65 (2009). PubMed: 19520781