Recombinant Anti-PLCG 2 (phospho Y753) antibody [EPR5914-3] (ab133455)
![Western blot - Anti-PLCG 2 (phospho Y753) antibody [EPR5914-3] (ab133455)](https://www.abcam.com/ps/products/133/ab133455/Images/ab133455-147083-anti-plcg-2-phospho-y753-antibody-epr5914-3-western-blot.jpg "Western blot - Anti-PLCG 2 (phospho Y753) antibody [EPR5914-3] (ab133455)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5914-3] to PLCG 2 (phospho Y753)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PLCG 2 (phospho Y753) antibody [EPR5914-3]
See all PLCG 2 primary antibodies -
Description
Rabbit monoclonal [EPR5914-3] to PLCG 2 (phospho Y753) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Raji cell lysate treated with Pervanadate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5914-3 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
|
| Notes |
|---|
|
WB
1/50000 - 1/200000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
-
Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity. - Information by UniProt
-
Database links
- Entrez Gene: 5336 Human
- Omim: 600220 Human
- SwissProt: P16885 Human
- Unigene: 413111 Human
-
Alternative names
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
see all
Images
-
Western blot - Anti-PLCG 2 (phospho Y753) antibody [EPR5914-3] (ab133455)All lanes : Anti-PLCG 2 (phospho Y753) antibody [EPR5914-3] (ab133455) at 1/50000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Raji cell lysate treated with Pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
![Anti-PLCG 2 (phospho Y753) antibody [EPR5914-3] (ab133455)](https://www.abcam.com/ps/products/133/ab133455/Images/ab133455-2-benefits-of-recombinant-antibodies.png)
Anti-PLCG 2 (phospho Y753) antibody [EPR5914-3] (ab133455)
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab133455 has been referenced in 1 publication.
- Duarte DP et al. Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms. Nat Commun 11:2319 (2020). PubMed: 32385234