Recombinant Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)
![Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)](https://www.abcam.com/ps/products/51/ab51020/Images/ab51020-62852-anti-plcg-2-phospho-y759-antibody-ep1406y-western-blot.jpg "Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759)
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-PLCG 2 (phospho Y759) antibody [EP1406Y]
See all PLCG 2 primary antibodies -
Description
Rabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1406Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
|
|
| WB | (1) |
1/50000 - 1/100000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
|
| Notes |
|---|
|
ICC/IF
1/50 - 1/100. |
|
WB
1/50000 - 1/100000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa). |
Target
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Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity. - Information by UniProt
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Database links
- Entrez Gene: 5336 Human
- Omim: 600220 Human
- SwissProt: P16885 Human
- Unigene: 413111 Human
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Alternative names
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
see all
Images
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Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)All lanes : Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020) at 1/100000 dilution
Lane 1 : Raji cell lysate, untreated
Lane 2 : Raji cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)](https://www.abcam.com/ps/products/51/ab51020/Images/ab51020-2-anti-plcg-2-antibody-ep1406y-immunocytochemistry-raji-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)Immunocytochemistry analysis of Raji (human Burkitt's lymphoma B lymphocyte) labeling PLCG 2 with purified ab51020 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.12 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab51020 has been referenced in 1 publication.
- Mohammad DK et al. Dual phosphorylation of Btk by Akt/protein kinase b provides docking for 14-3-3?, regulates shuttling, and attenuates both tonic and induced signaling in B cells. Mol Cell Biol 33:3214-26 (2013). PubMed: 23754751