Anti-PLK1 antibody [35-206] (ab17056)
![Immunocytochemistry - Anti-PLK1 antibody [35-206] (ab17056)](https://www.abcam.com/ps/products/17/ab17056/Images/ab17056-458701-anti-plk1-35206-immunocytochemistry-antibody-hela-human.jpg "Immunocytochemistry - Anti-PLK1 antibody [35-206] (ab17056)")
Key features and details
- Mouse monoclonal [35-206] to PLK1
- Suitable for: ICC, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-PLK1 antibody [35-206]
See all PLK1 primary antibodies -
Description
Mouse monoclonal [35-206] to PLK1 -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant full length protein corresponding to Human PLK1.
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Epitope
aa330-370. -
Positive control
- WB: A431, U-2 OS and HeLa S3 lysates. Flow cyt: HCT 116 cells. ICC/IF: HeLa cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
35-206 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 67 kDa). This clone is superior to clone 36-298 (ab17057) in Western blotting and IP, but suffers from high background in IF and ICC.
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| Notes |
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ICC
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 67 kDa). This clone is superior to clone 36-298 (ab17057) in Western blotting and IP, but suffers from high background in IF and ICC. |
Target
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Function
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. -
Tissue specificity
Placenta and colon. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
Developmental stage
Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. -
Post-translational
modificationsCatalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. - Information by UniProt
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Database links
- Entrez Gene: 5347 Human
- Entrez Gene: 25515 Rat
- Omim: 602098 Human
- SwissProt: P53350 Human
- SwissProt: Q62673 Rat
- Unigene: 592049 Human
- Unigene: 11034 Rat
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Alternative names
- Cell cycle regulated protein kinase antibody
- PLK 1 antibody
- PLK antibody
see all
Images
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Immunocytochemistry - Anti-PLK1 antibody [35-206] (ab17056)
ab264082 staining PLK1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab264082 at 1μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Western blot - Anti-PLK1 antibody [35-206] (ab17056)](https://www.abcam.com/ps/products/17/ab17056/Images/ab17056-25902-anti-plk1-antibody-35-206-western-blot.jpg)
Western blot - Anti-PLK1 antibody [35-206] (ab17056)Anti-PLK1 antibody [35-206] (ab17056) at 1 µg/ml + A431 cell lysate at 20 µg
Secondary
Rabbit-anti mouse Alexa fluor(680) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-PLK1 antibody [35-206] (ab17056)](https://www.abcam.com/ps/products/17/ab17056/Images/ab17056-25904-anti-plk1-antibody-35-206-western-blot.jpg)
Western blot - Anti-PLK1 antibody [35-206] (ab17056)All lanes : Anti-PLK1 antibody [35-206] (ab17056)
Lane 1 : Recombinant PLK1
Lane 2 : U2OS lysate
Lane 3 : HeLaS3
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?Western blot using ab17056.
Lane 1: recombinant PLK1
Lane 2: U2OS lysate
Lane 3: HeLaS3 lysate
15% SDS-PAGE gel -
![Immunocytochemistry - Anti-PLK1 antibody [35-206] (ab17056)](https://www.abcam.com/ps/products/17/ab17056/Images/ab17056-25901-anti-plk1-antibody-35-206-immunofluorescence.jpg)
Immunocytochemistry - Anti-PLK1 antibody [35-206] (ab17056)This image is courtesy of an Abreview submitted by Melanie Adlerab17056 at 1/100 staining human kidney tubular epithelial cells by ICC/IF. The cells were formaldehyde fixed, permeabilized with Triton X-100 and blocked with goat serum before incuabtion with the antibody. A goat anti-mouse FITC antibody was used as the secondary. -
![Flow Cytometry - Anti-PLK1 antibody [35-206] (ab17056)](https://www.abcam.com/ps/products/17/ab17056/Images/ab17056-25900-anti-plk1-antibody-35-206-flow-cytometry.jpg)
Flow Cytometry - Anti-PLK1 antibody [35-206] (ab17056)Overlay histogram showing HCT116 cells stained with ab17056 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17056, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab17056 has been referenced in 45 publications.
- Tatekawa S et al. N(6)-methyladenosine methylation-regulated polo-like kinase 1 cell cycle homeostasis as a potential target of radiotherapy in pancreatic adenocarcinoma. Sci Rep 12:11074 (2022). WB, IHC-P, ICC, Flow Cyt ; Human . PubMed: 35773310
- Xie G et al. Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines. STAR Protoc 2:100495 (2021). PubMed: 34195669
- Pal-Ghosh R et al. CDC2 Is an Important Driver of Vascular Smooth Muscle Cell Proliferation via FOXM1 and PLK1 in Pulmonary Arterial Hypertension. Int J Mol Sci 22:N/A (2021). PubMed: 34203295
- Zhang H et al. MicroRNA-545 suppresses progression of ovarian cancer through mediating PLK1 expression by a direct binding and an indirect regulation involving KDM4B-mediated demethylation. BMC Cancer 21:163 (2021). PubMed: 33588776
- Du N et al. Hsa_circRNA_102541 regulates the development of atherosclerosis by targeting miR-296-5p/PLK1 pathway. Ir J Med Sci N/A:N/A (2021). PubMed: 34251586