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  1. Link

    products/primary-antibodies/plvappv-1-antibody-1742-ab81719.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Endothelial
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Anti-PLVAP/PV-1 antibody [174/2] (ab81719)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (3)

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Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)
  • Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)

Key features and details

  • Mouse monoclonal [174/2] to PLVAP/PV-1
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Recombinant Human PLVAP/PV-1 protein (ab153539)
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Overview

  • Product name

    Anti-PLVAP/PV-1 antibody [174/2]
    See all PLVAP/PV-1 primary antibodies
  • Description

    Mouse monoclonal [174/2] to PLVAP/PV-1
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human tonsil cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab81719 is 0.2µM filtered.
  • Clonality

    Monoclonal
  • Clone number

    174/2
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human PLVAP/PV-1 protein (ab153539)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ICC/IF
Notes
Application notes
Flow Cyt: Typical starting dilution 1/50.
ICC/IF: Typical starting dilution 1/50.
IHC-Fr: Typical starting dilution 1/50.
WB: Typical starting dilution 1/50. Predicted molecular weight: 50 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Function

    Involved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability.
  • Tissue specificity

    Expressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium.
  • Cellular localization

    Cell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region.
  • Target information above from: UniProt accession Q9BX97 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 83483 Human
    • Omim: 607647 Human
    • SwissProt: Q9BX97 Human
    • Unigene: 107125 Human
    • Alternative names

      • FELS antibody
      • Fenestrated endothelial linked structure protein antibody
      • Fenestrated endothelial-linked structure protein antibody
      • gp68 antibody
      • Hypothetical protein DKFZp667O074 antibody
      • Meca 32 antigen antibody
      • MECA32 antigen antibody
      • Panendothelial cell antigen antibody
      • Plasmalemma vesicle associated protein antibody
      • Plasmalemma vesicle protein 1 antibody
      • Plasmalemma vesicle-associated protein antibody
      • Plvap antibody
      • PLVAP_HUMAN antibody
      • PV 1 antibody
      • PV-1 antibody
      • PV1 antibody
      • PV1 protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)
      Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)

      ab81719 at 1/50 dilution staining PLVAP/PV-1 in human tonsil by Immunofluorescence.

    • Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)
      Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)
      Overlay histogram showing HL60 cells stained with ab81719 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81719, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab81719? Please let us know so that we can cite the reference in this datasheet.

    ab81719 has been referenced in 3 publications.

    • Nakagami Y  et al. An anti-PLVAP antibody suppresses laser-induced choroidal neovascularization in monkeys. Eur J Pharmacol 854:240-246 (2019). PubMed: 31026444
    • Belle M  et al. Tridimensional Visualization and Analysis of Early Human Development. Cell 169:161-173.e12 (2017). PubMed: 28340341
    • Elkadri A  et al. Mutations in Plasmalemma Vesicle Associated Protein Result in Sieving Protein-Losing Enteropathy Characterized by Hypoproteinemia, Hypoalbuminemia, and Hypertriglyceridemia. Cell Mol Gastroenterol Hepatol 1:381-394.e7 (2015). PubMed: 26207260

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-PLVAP/PV-1 antibody [174/2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human embryonic pancreas PCW 10.4)
    Permeabilization
    Yes - Triton
    Specification
    Human embryonic pancreas PCW 10.4
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Aug 03 2021

    IHC - Wholemount abreview for Anti-PLVAP/PV-1 antibody [174/2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Embryo (Whole kidney)
    Specification
    Whole kidney
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Nov 23 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PLVAP/PV-1 antibody [174/2]

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (bone marrow)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval
    Permeabilization
    No
    Specification
    bone marrow
    Blocking step
    Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 17 2014

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