Anti-PLVAP/PV-1 antibody [174/2] (ab81719)
![Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)](https://www.abcam.com/ps/products/81/ab81719/Images/PLVAP-Primary-antibodies-ab81719-1.gif "Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)")
Key features and details
- Mouse monoclonal [174/2] to PLVAP/PV-1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PLVAP/PV-1 antibody [174/2]
See all PLVAP/PV-1 primary antibodies -
Description
Mouse monoclonal [174/2] to PLVAP/PV-1 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human tonsil cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab81719 is 0.2µM filtered. -
Clonality
Monoclonal -
Clone number
174/2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | ||
| ICC/IF |
| Notes |
|---|
Application notes
Flow Cyt: Typical starting dilution 1/50.
ICC/IF: Typical starting dilution 1/50.
IHC-Fr: Typical starting dilution 1/50.
WB: Typical starting dilution 1/50. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Typical starting dilution 1/50.
IHC-Fr: Typical starting dilution 1/50.
WB: Typical starting dilution 1/50. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Involved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability. -
Tissue specificity
Expressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium. -
Cellular localization
Cell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 83483 Human
- Omim: 607647 Human
- SwissProt: Q9BX97 Human
- Unigene: 107125 Human
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Alternative names
- FELS antibody
- Fenestrated endothelial linked structure protein antibody
- Fenestrated endothelial-linked structure protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)
ab81719 at 1/50 dilution staining PLVAP/PV-1 in human tonsil by Immunofluorescence.
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![Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)](https://www.abcam.com/ps/products/81/ab81719/Images/PLVAP-Primary-antibodies-ab81719-2.jpg)
Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)Overlay histogram showing HL60 cells stained with ab81719 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81719, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab81719 has been referenced in 6 publications.
- Song MJ et al. Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration. Nat Methods 20:149-161 (2023). PubMed: 36550275
- Muranyi W et al. Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in vitro model of the choroid plexus. iScience 25:104383 (2022). PubMed: 35633941
- Nakagami Y et al. An anti-PLVAP antibody suppresses laser-induced choroidal neovascularization in monkeys. Eur J Pharmacol 854:240-246 (2019). PubMed: 31026444
- Belle M et al. Tridimensional Visualization and Analysis of Early Human Development. Cell 169:161-173.e12 (2017). PubMed: 28340341
- Wisniewska-Kruk J et al. Plasmalemma Vesicle-Associated Protein Has a Key Role in Blood-Retinal Barrier Loss. Am J Pathol 186:1044-54 (2016). PubMed: 26878208
- Elkadri A et al. Mutations in Plasmalemma Vesicle Associated Protein Result in Sieving Protein-Losing Enteropathy Characterized by Hypoproteinemia, Hypoalbuminemia, and Hypertriglyceridemia. Cell Mol Gastroenterol Hepatol 1:381-394.e7 (2015). PubMed: 26207260