Recombinant Anti-PMCA1 antibody [EPR12029] (ab190355)
![Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-393588-anti-pmca1-antibody-epr12029-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12029] to PMCA1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PMCA1 antibody [EPR12029]
See all PMCA1 primary antibodies -
Description
Rabbit monoclonal [EPR12029] to PMCA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1, HeLa, Neuro-2a, U87-MG and HepG2 cell lysates. IHC-P: Human stomach and Human transitional cell carcinoma of bladder tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12029 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 130-250 kDa (predicted molecular weight: 139 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 130-250 kDa (predicted molecular weight: 139 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. -
Tissue specificity
Isoform B is ubiquitously expressed. Isoform C is found in brain cortex, skeletal muscle and heart muscle. Isoform D has only been found in fetal skeletal muscle. Isoform K has been found in small intestine and liver. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. -
Domain
The calmodulin-binding subdomain B is different in the different splice variants and shows pH dependent calmodulin binding properties in isoforms A, C, D and E. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 490 Human
- Entrez Gene: 67972 Mouse
- Entrez Gene: 29598 Rat
- Omim: 108731 Human
- SwissProt: P20020 Human
- SwissProt: P11505 Rat
- Unigene: 506276 Human
- Unigene: 7208 Rat
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Alternative names
- AT2B1_HUMAN antibody
- ATP2B1 antibody
- ATPase Ca++ transporting plasma membrane 1 antibody
see all
Images
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Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ATP2B1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 139 kDa
Observed band size: 180-245 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab190355 observed at 180-245 kDa. Red - loading control ab8245 observed at 36 kDa.
ab190355 Anti-PMCA1 antibody [EPR12029] was shown to specifically react with PMCA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265561 (knockout cell lysate ab257365) was used. Wild-type and PMCA1 knockout samples were subjected to SDS-PAGE. ab190355 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-226270-ab190355c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-226271-ab190355d.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMCA1 antibody [EPR12029] (ab190355)Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-226269-ab190355b.jpg)
Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-226268-ab190355.jpg)
Western blot - Anti-PMCA1 antibody [EPR12029] (ab190355)All lanes : Anti-PMCA1 antibody [EPR12029] (ab190355) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 139 kDa
Observed band size: 130-250 kDa why is the actual band size different from the predicted? -
![Anti-PMCA1 antibody [EPR12029] (ab190355)](https://www.abcam.com/ps/products/190/ab190355/Images/ab190355-6-benefits-of-recombinant-antibodies.png)
Anti-PMCA1 antibody [EPR12029] (ab190355)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab190355 has been referenced in 10 publications.
- Rodrat M et al. Vasoactive intestinal peptide and cystic fibrosis transmembrane conductance regulator contribute to the transepithelial calcium transport across intestinal epithelium-like Caco-2 monolayer. PLoS One 17:e0277096 (2022). PubMed: 36399482
- Ilic K et al. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts. Int J Mol Sci 22:N/A (2021). PubMed: 34948386
- Dharmaraj P et al. Neonatal Hypocalcemic Seizures in Offspring of a Mother With Familial Hypocalciuric Hypercalcemia Type 1 (FHH1). J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32150253
- Bathala P et al. Oviductal extracellular vesicles (oviductosomes, OVS) are conserved in humans: murine OVS play a pivotal role in sperm capacitation and fertility. Mol Hum Reprod 24:143-157 (2018). PubMed: 29370405
- Fereshteh Z et al. Detection of extracellular vesicles in the mouse vaginal fluid: Their delivery of sperm proteins that stimulate capacitation and modulate fertility. J Cell Physiol N/A:N/A (2018). PubMed: 30536802
- Gorvin CM et al. A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate ß-arrestin-biased signaling. Sci Signal 11:N/A (2018). PubMed: 29463778
- Gorvin CM et al. Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias. Hum Mol Genet 27:3720-3733 (2018). PubMed: 30052933
- Frandsen SK et al. Normal and Malignant Cells Exhibit Differential Responses to Calcium Electroporation. Cancer Res 77:4389-4401 (2017). PubMed: 28760856
- Robertson SYT et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . PubMed: 28588505
- Chu W et al. Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B. Exp Ther Med 12:1741-1749 (2016). PubMed: 27602089