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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)

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Western blot - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
  • Western blot - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
  • Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5614] to PMP70 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-PMP70 antibody [EPR5614] - BSA and Azide free
    See all PMP70 primary antibodies

  • Description

    Rabbit monoclonal [EPR5614] to PMP70 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HAP1, A431 and HepG2 cell lysates.

  • General notes

    ab247868 is the carrier-free version of ab109448.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.

  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.

  • Cellular localization

    Peroxisome membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 70 kDa peroxisomal membrane protein antibody
    • ABC 43 antibody
    • ABC D3 antibody
    • ABC43 antibody
    • ABCD 3 antibody
    • Abcd3 antibody
    • ABCD3 protein antibody
    • ABCD3_HUMAN antibody
    • ATP binding cassette sub family D (ALD) member 3 antibody
    • ATP binding cassette sub family D member 3 antibody
    • ATP-binding cassette sub-family D member 3 antibody
    • CBAS5 antibody
    • dJ824O18.1 (ATP-binding cassette, sub family D (ALD), member 3 (PMP70, PXMP1)) antibody
    • Peroxisomal membrane protein 1 (70kD) antibody
    • Peroxisomal membrane protein 1 (70kD, Zellweger syndrome) antibody
    • Peroxisomal membrane protein 1 antibody
    • PMP 70 antibody
    • PMP70 antibody
    • PXMP 1 antibody
    • PXMP1 antibody
    • ZWS2 antibody
    see all

Images

  • Western blot - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
    Western blot - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
    All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ABCD3 knockout HeLa cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 75 kDa
    Observed band size: 75 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab109448).

      Lanes 1- 2: Merged signal (red and green). Green - ab109448 observed at 75 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab109448 was shown to react with PMP70 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265294 (knockout cell lysate ab257134) was used. Wild-type HeLa and PMP70 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109448 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
    Western blot - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
    All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ABCD3 (PMP70) knockout HAP1 whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 75 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab109448).

    Lanes 1 - 4: Merged signal (red and green). Green - ab109448 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109448 was shown to specifically react with PMP70 in wild-type HAP1 cells as signal was lost in ABCD3 (PMP70) knockout cells. Wild-type and ABCD3 (PMP70) knockout samples were subjected to SDS-PAGE. Ab109448 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
    Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling PMP70 with primary antibody anti-PMP70 (ab109448) at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150081) secondary antibody at 1/1000 dilution (2.0 µg/mL). Confocal image showing cytoplasmic staining on HeLa cells. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL). The nuclear counter stain is DAPI (blue).
    The secondary antibody only control is : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2.0 µg/mL).

    This data was developed using the same antibody clone in a different buffer formulation (ab109448). 

  • Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)
    Anti-PMP70 antibody [EPR5614] - BSA and Azide free (ab247868)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab247868? Please let us know so that we can cite the reference in this datasheet.

ab247868 has not yet been referenced specifically in any publications.

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