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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-POLDIP2 antibody [EPR13832] (ab181841)

Reviews (1) Submit a question References (7)
Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
  • Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] (ab181841)
  • Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
  • Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (ab181841)
  • Anti-POLDIP2 antibody [EPR13832] (ab181841)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13832] to POLDIP2
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-POLDIP2 antibody [EPR13832]
    See all POLDIP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13832] to POLDIP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HepG2 cell lysates. Flow Cyt (intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13832

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/350.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa).
IP
1/40.
Notes
Flow Cyt (Intra)
1/350.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa).
IP
1/40.

Target

Images

  • Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: POLDIP2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181841 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab181841 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when POLDIP2 knockout samples were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab181841 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

     

  • Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Flow Cytometry (Intracellular) - Anti-POLDIP2 antibody [EPR13832] (ab181841)

    Intracellular flow cytometric analysis of2% paraformaldehyde-fixed HeLa cells labeling POLDIP2 with ab181841 at 1/350 diltuion (red) compared to aRabbit monoclonal IgG Isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. 

     

     

  • Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    All lanes : Anti-POLDIP2 antibody [EPR13832] (ab181841) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (ab181841)

    Western blot analysis of HeLa cell lysate immunoprecipitated with ab181841 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.

  • Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Anti-POLDIP2 antibody [EPR13832] (ab181841)

References (7)

Publishing research using ab181841? Please let us know so that we can cite the reference in this datasheet.

ab181841 has been referenced in 7 publications.

  • Eidson LN  et al. Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction. Sci Rep 11:5533 (2021). PubMed: 33692398
  • Gao M  et al. Poldip2 mediates blood-brain barrier disruption and cerebral edema by inducing AQP4 polarity loss in mouse bacterial meningitis model. CNS Neurosci Ther 26:1288-1302 (2020). PubMed: 32790044
  • Forrester SJ  et al. Poldip2 deficiency protects against lung edema and vascular inflammation in a model of acute respiratory distress syndrome. Clin Sci (Lond) 133:321-334 (2019). PubMed: 30622219
  • Kikuchi DS  et al. Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathy. J Neuroinflammation 16:241 (2019). PubMed: 31779628
  • Huff LP  et al. Polymerase-d-interacting protein 2 activates the RhoGEF epithelial cell transforming sequence 2 in vascular smooth muscle cells. Am J Physiol Cell Physiol 316:C621-C631 (2019). PubMed: 30726115
  • Hernandes MS  et al. Polymerase delta-interacting protein 2 deficiency protects against blood-brain barrier permeability in the ischemic brain. J Neuroinflammation 15:45 (2018). PubMed: 29452577
  • Li MS  et al. NADPH oxidase-2 mediates zinc deficiency-induced oxidative stress and kidney damage. Am J Physiol Cell Physiol 312:C47-C55 (2017). PubMed: 27806940

Customer reviews and Q&As

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Western blot abreview for Anti-POLDIP2 antibody [EPR13832]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells)
Gel Running Conditions
Reduced Denaturing (12 % gel)
Loading amount
1 µg
Specification
293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ko Suzuki

Verified customer

Submitted Jul 26 2019

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