Anti-POLR3A antibody (ab247007)
Key features and details
- Rabbit polyclonal to POLR3A
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-POLR3A antibody
See all POLR3A primary antibodies -
Description
Rabbit polyclonal to POLR3A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken, Cow
-
Immunogen
Recombinant fragment corresponding to Human POLR3A aa 600-700.
Database link: O14802 -
Positive control
- WB: PC3 and MCF7 whole cell lysates. IHC-P: Human cerebral cortex, testis, placenta and liver tissue. ICC/IF: U-2 OS cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 156 kDa.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| Notes |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 156 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
-
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. -
Tissue specificity
Expressed in the brain, in the cortex and the white matter (at protein level). -
Involvement in disease
Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism -
Sequence similarities
Belongs to the RNA polymerase beta' chain family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 11128 Human
- Omim: 614258 Human
- SwissProt: O14802 Human
- Unigene: 436896 Human
-
Alternative names
- BC053071 antibody
- DNA directed RNA polymerase III largest subunit antibody
- DNA directed RNA polymerase III subunit A antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-POLR3A antibody (ab247007)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for POLR3A (green) using ab247007 at 4 μg/ml in ICC/IF.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3A antibody (ab247007)Paraffin-embedded human liver tissue stained for POLR3A using ab247007 at 1/200 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3A antibody (ab247007)Paraffin-embedded human placenta tissue stained for POLR3A using ab247007 at 1/200 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3A antibody (ab247007)Paraffin-embedded human testis tissue stained for POLR3A using ab247007 at 1/200 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLR3A antibody (ab247007)Paraffin-embedded human cerebral cortex tissue stained for POLR3A using ab247007 at 1/200 dilution in immunohistochemical analysis. -
Western blot - Anti-POLR3A antibody (ab247007)All lanes : Anti-POLR3A antibody (ab247007) at 0.4 µg/ml
Lane 1 : PC-3 whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 156 kDaCorresponding POLR3A RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
-
Western blot - Anti-POLR3A antibody (ab247007)Anti-POLR3A antibody (ab247007) at 0.4 µg/ml + PC-3 whole cell lysate
Predicted band size: 156 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab247007 has not yet been referenced specifically in any publications.