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RecombinantRabMAb

Recombinant Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

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Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
  • Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
  • Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
  • Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
  • Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
  • Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
  • Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21993-154] to Polyethylene glycol
  • Suitable for: WB, ELISA, Sandwich ELISA
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Polyethylene glycol antibody [EPR21993-154]
    See all Polyethylene glycol primary antibodies

  • Description

    Rabbit monoclonal [EPR21993-154] to Polyethylene glycol

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISA, Sandwich ELISAmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: BSA-methoxy PEG 5K lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21993-154

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000.
ELISA
1/100.
Sandwich ELISA
Use at an assay dependent concentration.
Notes
WB
1/1000.
ELISA
1/100.
Sandwich ELISA
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-P.

Target

  • Alternative names

    • PEG antibody

Images

  • Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/1000 dilution + BSA-methoxy PEG 5K, 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 250 kDa why is the actual band size different from the predicted?



    Exposure time: 92 seconds.

    Blocking/Diluting buffer: 5% NFDM/TBST.

  • Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using AffniPure Goat Anti-Mouse IgG (H&L) at 1 µg/ml concentration. The detector antibody was ab203857 anti-Polyethylene glycol at 1 µg/ml. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Antigen concentration range: 0-1000 ng/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: p-nitrophenyl phosphate (PNPP).

    Coloration time: 10 min.

  • Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

    Antigen concentration range: 0-1000 ng/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).

    Coloration time: 2 min.

  • Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

    Antigen concentration range: 0-1000 ng/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).

    Coloration time: 2 min.

  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Antigen concentration range: 0-0.05µg/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: p-nitrophenyl phosphate (PNPP).

    Coloration time: 15 min.

  • ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

    Antigen concentration range: 0-0.05µg/ml.

    Blocking/Diluting Buffer and concentration: 1% BSA/TBS.

    Washing Buffer: 1 X TBST.

    Substrate: p-nitrophenyl phosphate (PNPP).

    Coloration time: 15 min.

  • Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Method: APS sensor+ Antigen (methoxy PEG800 at 0.625 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 2, 1, 0.5, 0.25, 0.125µg/ml).

    Results:

    KD (M) = 5.81E-11

    kon(1/Ms) = 2.70E+05

    kon Error = 6.28E+02

    kdis(1/s) = 1.57E-05

    kdis Error = 4.49E-07

    Full X2= 10.143

    Full R2= 0.9993

    Testing Biosensor: Aminopropylsilane (APS) Biosensors

    Testing machine: Fortebio RED96e

  • Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

    Method: APS sensor+ Antigen (methoxy PEG800 at 25 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 0.5, 0.25, 0.125, 0.0625, 0.03125µg/ml).

    Results:

    KD (M) = 1.29E-10

    kon(1/Ms) = 2.23E+06

    kon Error = 1.45E+04

    kdis(1/s) = 2.88E-04

    kdis Error = 1.74E-06

    Full X2 = 3.475

    Full R2 = 0.9865

    Testing Biosensor: Aminopropylsilane (APS) Biosensors

    Testing machine: Fortebio RED96e

  • Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)
    Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab203857? Please let us know so that we can cite the reference in this datasheet.

ab203857 has not yet been referenced specifically in any publications.

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