Recombinant Anti-POMC antibody [EPR17571] (ab210605)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266376-anti-pomc-antibody-epr17571-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17571] to POMC
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-POMC antibody [EPR17571]
See all POMC primary antibodies -
Description
Rabbit monoclonal [EPR17571] to POMC -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with POMC whole cell lysate; rat and mouse pituitary lysates; human ACTH recombinant protein. IHC-P: Human, mouse and rat pituitary tissues. IP: Rat pituitary whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17571 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/80.
|
|
| IHC-P |
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/5000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| Notes |
|---|
|
IP
1/80. |
|
IHC-P
1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/5000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 5443 Human
- Entrez Gene: 18976 Mouse
- Entrez Gene: 24664 Rat
- Omim: 176830 Human
- SwissProt: P01189 Human
- SwissProt: P01193 Mouse
- SwissProt: P01194 Rat
- Unigene: 1897 Human
see all -
Alternative names
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on mouse pituitary is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266374-anti-pomc-antibody-epr17571-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)Immunohistochemical analysis of paraffin-embedded rat pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on rat pituitary is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266380-anti-pomc-antibody-epr17571-western-blot.jpg)
Western blot - Anti-POMC antibody [EPR17571] (ab210605)Anti-POMC antibody [EPR17571] (ab210605) at 1/1000 dilution + human ACTH recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Human ACTH recombinant peptide contains 3*aa138-176 with His-tag.
This product is capable of recognizing the ACTH peptide.
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![Western blot - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266381-anti-pomc-antibody-epr17571-western-blot.jpg)
Western blot - Anti-POMC antibody [EPR17571] (ab210605)Anti-POMC antibody [EPR17571] (ab210605) at 1/5000 dilution + mouse pituitary lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266382-anti-pomc-antibody-epr17571-western-blot.jpg)
Western blot - Anti-POMC antibody [EPR17571] (ab210605)Anti-POMC antibody [EPR17571] (ab210605) at 1/1000 dilution + rat pituitary lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266842-anti-pomc-antibody-epr17571-immunoprecipitation.jpg)
Immunoprecipitation - Anti-POMC antibody [EPR17571] (ab210605)ab210605 at 1/80 immunoprecipitating POMC in Rat pituitary whole cell lysate observed at 29 KDa (lanes 1 and 2).
Lane 1 (input): Rat pituitary whole cell lysate 10μg
Lane 2 (+): ab210605 + Rat pituitary whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab210605 in Rat pituitary whole cell lysate
For western blotting, ab210605 at 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266379-anti-pomc-antibody-epr17571-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)Immunohistochemical analysis of paraffin-embedded human pituitary tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human pituitary is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266384-anti-pomc-antibody-epr17571-western-blot.jpg)
Western blot - Anti-POMC antibody [EPR17571] (ab210605)All lanes : Anti-POMC antibody [EPR17571] (ab210605) at 1/10000 dilution
Lane 1 : Untransfected HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with POMC whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266375-anti-pomc-antibody-epr17571-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative staining on mouse kidney.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266383-anti-pomc-antibody-epr17571-western-blot.jpg)
Western blot - Anti-POMC antibody [EPR17571] (ab210605)Anti-POMC antibody [EPR17571] (ab210605) at 1/5000 dilution + rat pituitary lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266378-anti-pomc-antibody-epr17571-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative staining on human kidney.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266377-anti-pomc-antibody-epr17571-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative staining on human colon cancer.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-266373-anti-pomc-antibody-epr17571-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMC antibody [EPR17571] (ab210605)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling POMC with ab210605 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative staining on rat liver.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-POMC antibody [EPR17571] (ab210605)](https://www.abcam.com/ps/products/210/ab210605/Images/ab210605-15-benefits-of-recombinant-antibodies.png)
Anti-POMC antibody [EPR17571] (ab210605)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab210605 has been referenced in 8 publications.
- Gu WT et al. A potential impact of SARS-CoV-2 on pituitary glands and pituitary neuroendocrine tumors. Endocrine 72:340-348 (2021). PubMed: 33786714
- Ye L et al. C1q/TNF-related protein 4 restores leptin sensitivity by downregulating NF-?B signaling and microglial activation. J Neuroinflammation 18:159 (2021). PubMed: 34275474
- Wang A et al. The genomic profiling and MAMLD1 expression in human and canines with Cushing's disease. BMC Endocr Disord 21:185 (2021). PubMed: 34517852
- Liao T et al. Liraglutide Lowers Body Weight Set Point in DIO Rats and its Relationship with Hypothalamic Microglia Activation. Obesity (Silver Spring) 28:122-131 (2020). PubMed: 31773909
- Song L et al. Maternal exercise during gestation and lactation decreases high-fat diet preference by altering central reward system gene expression in adult female offspring from high-fat fed dams. Behav Brain Res 390:112660 (2020). PubMed: 32387350
- Chasseloup F et al. Germline CDKN1B Loss-of-Function Variants Cause Pediatric Cushing's Disease With or Without an MEN4 Phenotype. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32232325
- Chen Y et al. Constant light exposure aggravates POMC-mediated muscle wasting associated with hypothalamic alteration of circadian clock and SIRT1 in endotoxemia rats. Biochem Biophys Res Commun 508:811-817 (2019). PubMed: 30528733
- Cao C et al. Hypothalamic AMPK-induced autophagy ameliorates hypercatabolism in septic rats by regulating POMC expression. Biochem Biophys Res Commun 497:1089-1096 (2018). WB . PubMed: 29496447