Anti-POU Domain 2 antibody [6G6AC11] (ab201325)
![Immunocytochemistry/ Immunofluorescence - Anti-POU Domain 2 antibody [6G6AC11] (ab201325)](https://www.abcam.com/ps/products/201/ab201325/Images/ab201325-242845-anti-pou-domain-2-antibody-6g6ac11-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-POU Domain 2 antibody [6G6AC11] (ab201325)")
Key features and details
- Rat monoclonal [6G6AC11] to POU Domain 2
- Suitable for: ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG2a
Overview
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Product name
Anti-POU Domain 2 antibody [6G6AC11] -
Description
Rat monoclonal [6G6AC11] to POU Domain 2 -
Host species
Rat -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Drosophila stage 10 embryos.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notes
Purity: 97.47% -
Clonality
Monoclonal -
Clone number
6G6AC11 -
Isotype
IgG2a -
Light chain type
kappa
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
DNA-binding regulatory protein implicated in early development. Involved in neuronal cell fate decision. May act as an octamer-dependent activator of transcription. Could also play an early role in specific ectodermal cells, and a subsequent role in the embryonic nervous system. Expressed primarily during the first half of embryogenesis. -
Cellular localization
nucleus -
Database links
- Entrez Gene: 34673 Drosophila melanogaster
- SwissProt: Q9VK71 Drosophila melanogaster
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Alternative names
- CG12287 antibody
- CG15486 antibody
- CG15487 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-POU Domain 2 antibody [6G6AC11] (ab201325)
Immunocytochemistry analysis in neuroblast nuclei from Drosophila stage 10 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum. The POU Domain 2 stain used ab201325 at 9 µg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-rat at 1:400.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab201325 has been referenced in 2 publications.
- Seroka A et al. A novel temporal identity window generates alternating Eve+/Nkx6+ motor neuron subtypes in a single progenitor lineage. Neural Dev 15:9 (2020). PubMed: 32723364
- Bahrampour S et al. Brain expansion promoted by polycomb-mediated anterior enhancement of a neural stem cell proliferation program. PLoS Biol 17:e3000163 (2019). PubMed: 30807568
