Recombinant Anti-PP1C gamma antibody [EPR8934] (ab134947)
![Western blot - Anti-PP1C gamma antibody [EPR8934] (ab134947)](https://www.abcam.com/ps/products/134/ab134947/Images/ab134947-391466-anti-protein-phosphatase-1-gamma-2-antibody-epr8934-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PP1C gamma antibody [EPR8934] (ab134947)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8934] to PP1C gamma
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PP1C gamma antibody [EPR8934]
See all PP1C gamma primary antibodies -
Description
Rabbit monoclonal [EPR8934] to PP1C gamma -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, HeLa, SKBR3 and T47D cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Dissociation constant (KD)
KD = 4.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8934 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
Target
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Function
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus speckle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. Colocalizes with SPZ1 in the nucleus (By similarity). Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleolus to nuclear speckles. Shows a dynamic targeting to specific sites throughout the cell cycle. Highly concentrated in nucleoli of interphase cells and localizes at kinetochores early in mitosis. Relocalization to chromosome-containing regions occurs at the transition from early to late anaphase. Also accumulates at the cleavage furrow and midbody by telophase. - Information by UniProt
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Database links
- Entrez Gene: 5501 Human
- Entrez Gene: 19047 Mouse
- Entrez Gene: 24669 Rat
- Omim: 176914 Human
- SwissProt: P36873 Human
- SwissProt: P63087 Mouse
- SwissProt: P63088 Rat
- Unigene: 79081 Human
see all -
Alternative names
- PP 1G antibody
- PP-1G antibody
- PP1C antibody
see all
Images
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Western blot - Anti-PP1C gamma antibody [EPR8934] (ab134947)All lanes : Anti-PP1C gamma antibody [EPR8934] (ab134947) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PPP1CC knockout HeLa cell lysate
Lane 3 : T-47D cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab134947 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.
ab134947 Anti-Protein phosphatase 1 gamma 2 antibody was shown to specifically react with Protein phosphatase 1 gamma 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264875 (knockout cell lysate ab258131) was used. Wild-type and Protein phosphatase 1 gamma 2 knockout samples were subjected to SDS-PAGE. ab134947 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![OI-RD Scanning - Anti-PP1C gamma antibody [EPR8934] (ab134947)](https://www.abcam.com/ps/products/134/ab134947/Images/ab134947-149154-anti-protein-phosphatase-1-gamma-2-antibody-epr8934.jpg)
OI-RD Scanning - Anti-PP1C gamma antibody [EPR8934] (ab134947) -
![Western blot - Anti-PP1C gamma antibody [EPR8934] (ab134947)](https://www.abcam.com/ps/products/134/ab134947/Images/ab134947-149155-anti-protein-phosphatase-1-gamma-2-antibody-epr8934-western-blot.jpg)
Western blot - Anti-PP1C gamma antibody [EPR8934] (ab134947)All lanes : Anti-PP1C gamma antibody [EPR8934] (ab134947) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SKBR3 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa -
![Anti-PP1C gamma antibody [EPR8934] (ab134947)](https://www.abcam.com/ps/products/134/ab134947/Images/ab134947-4-benefits-of-recombinant-antibodies.png)
Anti-PP1C gamma antibody [EPR8934] (ab134947)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab134947 has been referenced in 2 publications.
- Cai J et al. Simultaneous overactivation of Wnt/ß-catenin and TGFß signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC. Nat Commun 8:15870 (2017). PubMed: 28627514
- Garbacz WG et al. Hepatic Overexpression of CD36 Improves Glycogen Homeostasis and Attenuates High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance. Mol Cell Biol 36:2715-2727 (2016). PubMed: 27528620