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    products/primary-antibodies/pp2a-alpha--beta-antibody-y119-ab32141.pdf

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RecombinantRabMAb

Recombinant Anti-PP2A alpha + beta antibody [Y119] (ab32141)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (26)

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Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)
  • Flow Cytometry (Intracellular) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)
  • Anti-PP2A alpha + beta antibody [Y119] (ab32141)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y119] to PP2A alpha + beta
  • Suitable for: WB, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

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Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-PP2A alpha + beta antibody [Y119]
    See all PP2A alpha + beta primary antibodies
  • Description

    Rabbit monoclonal [Y119] to PP2A alpha + beta
  • Host species

    Rabbit
  • Specificity

    This antibody detects both alpha and beta isoforms of PP2A.
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    ab32141 reacts with an epitope located in the C terminal region of PP2A alpha and beta
  • Positive control

    • T47-D cell lysate and human breast carcinoma
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y119
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Troponin

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-PP2A alpha + beta antibody [Y119] (ab203702)
    • Alexa Fluor® 488 Anti-PP2A alpha + beta antibody [Y119] (ab203957)
    • Anti-PP2A alpha + beta antibody [Y119] - BSA and Azide free (ab247238)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/5000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/20.
Notes
WB
1/5000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/20.

Target

  • Function

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Post-translational
    modifications

    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Target information above from: UniProt accession P67775 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5515 Human
    • Entrez Gene: 5516 Human
    • Entrez Gene: 19052 Mouse
    • Entrez Gene: 19053 Mouse
    • Entrez Gene: 24672 Rat
    • Entrez Gene: 24673 Rat
    • Omim: 176915 Human
    • Omim: 176916 Human
    • SwissProt: P62714 Human
    • SwissProt: P67775 Human
    • SwissProt: P62715 Mouse
    • SwissProt: P63330 Mouse
    • SwissProt: P62716 Rat
    • SwissProt: P63331 Rat
    • Unigene: 105818 Human
    • Unigene: 260288 Mouse
    • Unigene: 1271 Rat
    see all
  • Alternative names

    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2A-beta antibody
    • PP2AA_HUMAN antibody
    • PP2Aalpha antibody
    • PP2AB_HUMAN antibody
    • PP2Abeta antibody
    • PP2Ac antibody
    • PP2CA antibody
    • PP2Calpha antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Protein phosphatase type 2A catalytic subunit antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • RPC antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

Images

  • Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)
    Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)
    Anti-PP2A alpha + beta antibody [Y119] (ab32141) at 1/10000 dilution + T47-D cell lysate

    Predicted band size: 35 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)

    ab32141,at a 1/250 dilution, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)
    Flow Cytometry (Intracellular) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling PP2A alpha + beta (red) with unpurified ab32141 at dilution of 1/20. Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibodies. 

     

     

  • Anti-PP2A alpha + beta antibody [Y119] (ab32141)
    Anti-PP2A alpha + beta antibody [Y119] (ab32141)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (26)

Publishing research using ab32141? Please let us know so that we can cite the reference in this datasheet.

ab32141 has been referenced in 26 publications.

  • Liang ZW  et al. Tumor-associated macrophages promote the metastasis and growth of non-small-cell lung cancer cells through NF-?B/PP2Ac-positive feedback loop. Cancer Sci 112:2140-2157 (2021). PubMed: 33609307
  • Gergs U  et al. Influence of Serotonin 5-HT4 Receptors on Responses to Cardiac Stressors in Transgenic Mouse Models. Biomedicines 9:N/A (2021). PubMed: 34070090
  • Singaravelu R  et al. A conserved miRNA-183 cluster regulates the innate antiviral response. J Biol Chem 294:19785-19794 (2019). PubMed: 31694919
  • Søgaard D  et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). PubMed: 30871020
  • da Silva RA  et al. Laminar shear stress-provoked cytoskeletal changes are mediated by epigenetic reprogramming of TIMP1 in human primary smooth muscle cells. J Cell Physiol 234:6382-6396 (2019). PubMed: 30238981
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse testis)
Loading amount
25 µg
Specification
Mouse testis
Gel Running Conditions
Reduced Denaturing (15 %)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Jul 18 2011

Immunoprecipitation abreview for Anti-PP2A alpha and beta antibody [Y119]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mouse testis)
Total protein in input
25 µg
Specification
mouse testis
Immuno-precipitation step
Protein G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Jul 14 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-PP2A alpha and beta antibody [Y119]

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT1080 Cell line)
Specification
HT1080 Cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 15 2010

Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 Cell line)
Loading amount
50000 cells
Specification
HT1080 Cell line
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 04 2010

Western blot abreview for Anti-PP2A alpha and beta antibody [Y119]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 lung carcinoma)
Loading amount
40 µg
Specification
A549 lung carcinoma
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 06 2009

Question

Product code: 32141

Inquiry: Hello, I'm working with filamentous fungi Neurospora crassa, I'd like to order antibody for protein phosphatase PP2A catalytic subunit. Visiting your website I saw a list of anti pp2a antibodies but my concern is that those antibodies will not link to Neurospora crassa protein PP2A as there are some differences in peptide sequence between human PP2A and Neurospora crassa PP2A catalytic subunit. I would appreciate your advice on this issue.

Read More

Abcam community

Verified customer

Asked on May 14 2012

Answer

Thank you for your inquiry.

We generally expect a cross reaction between species of an antibody when the homology is 85% and higher. We consider this then worth testing.

Unfortunately, the immunogenof ab32141 and Neurospora crassa only show 78% homology. Therefore I cannot recommend this antibody for your purposes.

I also check our other Anti-PP2A alpha and beta antibodies.

They all also show a homology that is too low to expect a cross reaction, apart fromab1309.

ab1309 shows a 84% homology with Neurospora crassa. This is borderline, but might be worth testing.

Therefore, I can offer a discount off a future purchase if you buy ab1309 now, test it in Neurosporacrassaand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody or the value off your next order.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab1309 in Neurospora crassa. I will then send a discount code. This code must be issued before purchasing ab1309 so please wait for my reply before ordering.

2. Purchase ab1309 either by phone, fax, or online (www.abcam.com).

3. Test it in Neurospora crassa.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab1309 turns out to be unsuitable for Neurospora crassa, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on May 14 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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