Recombinant Anti-PP2A alpha + beta antibody [Y119] (ab32141)
![Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)](https://www.abcam.com/ps/products/32/ab32141/Images/ab32141-44016-anti-pp2a-alpha-beta-antibody-y119-western-blot.jpg "Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y119] to PP2A alpha + beta
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PP2A alpha + beta antibody [Y119]
See all PP2A alpha + beta primary antibodies -
Description
Rabbit monoclonal [Y119] to PP2A alpha + beta -
Host species
Rabbit -
Specificity
This antibody detects both alpha and beta isoforms of PP2A. -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Epitope
ab32141 reacts with an epitope located in the C terminal region of PP2A alpha and beta -
Positive control
- T47-D cell lysate and human breast carcinoma
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y119 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/5000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| Flow Cyt (Intra) |
1/20.
|
| Notes |
|---|
|
WB
1/5000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
1/20. |
Target
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Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily. -
Post-translational
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
Cellular localization
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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Database links
- Entrez Gene: 5515 Human
- Entrez Gene: 5516 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 19053 Mouse
- Entrez Gene: 24672 Rat
- Entrez Gene: 24673 Rat
- Omim: 176915 Human
- Omim: 176916 Human
see all -
Alternative names
- PP2A A antibody
- PP2A alpha antibody
- PP2A B antibody
see all
Images
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Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)Anti-PP2A alpha + beta antibody [Y119] (ab32141) at 1/10000 dilution + T47-D cell lysate
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)](https://www.abcam.com/ps/products/32/ab32141/Images/ab32141-44015-anti-pp2a-alpha-beta-antibody-y119-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)ab32141,at a 1/250 dilution, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)](https://www.abcam.com/ps/products/32/ab32141/Images/ab32141-270741-anti-pp2a-alpha-beta-antibody-y119-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling PP2A alpha + beta (red) with unpurified ab32141 at dilution of 1/20. Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibodies.
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![Anti-PP2A alpha + beta antibody [Y119] (ab32141)](https://www.abcam.com/ps/products/32/ab32141/Images/ab32141-4-benefits-of-recombinant-antibodies.png)
Anti-PP2A alpha + beta antibody [Y119] (ab32141)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab32141 has been referenced in 26 publications.
- Liang ZW et al. Tumor-associated macrophages promote the metastasis and growth of non-small-cell lung cancer cells through NF-?B/PP2Ac-positive feedback loop. Cancer Sci 112:2140-2157 (2021). PubMed: 33609307
- Gergs U et al. Influence of Serotonin 5-HT4 Receptors on Responses to Cardiac Stressors in Transgenic Mouse Models. Biomedicines 9:N/A (2021). PubMed: 34070090
- Singaravelu R et al. A conserved miRNA-183 cluster regulates the innate antiviral response. J Biol Chem 294:19785-19794 (2019). PubMed: 31694919
- Søgaard D et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). PubMed: 30871020
- da Silva RA et al. Laminar shear stress-provoked cytoskeletal changes are mediated by epigenetic reprogramming of TIMP1 in human primary smooth muscle cells. J Cell Physiol 234:6382-6396 (2019). PubMed: 30238981