Recombinant Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)](https://www.abcam.com/ps/products/178/ab178865/Images/ab178865-258151-anti-ppar-alpha-ppar-beta-delta-antibody-epr166732-western-blot.jpg "Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)]
See all PPAR alpha + PPAR beta/delta primary antibodies -
Description
Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human PPAR alpha and delta full length recombinant proteins; Human fetal liver, fetal heart and fetal kidney lysates; Human skeletal muscle lysate; Jurkat, HeLa, K562, HepG2, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse placenta, brain, heart, kidney and spleen lysates; Rat placenta, brain, heart and kidney lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16673(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 52, 50 kDa).
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| Notes |
|---|
|
WB
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 52, 50 kDa). |
Images
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Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/10000 dilution
Lane 1 : Human PPAR alpha full length recombinant protein
Lane 2 : Human PPAR beta full length recombinant protein
Lane 3 : Human PPAR gamma fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52, 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 1 second; Lane 3: 3 minutes.
Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR beta full length recombinant protein contains aa1-441 with a His-Tag; Human PPAR gamma fragment recombinant protein contains aa1-200 with a His-Tag. These three recombinant proteins were made in-house.
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![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)](https://www.abcam.com/ps/products/178/ab178865/Images/ab178865-258149-anti-ppar-alpha-ppar-beta-delta-antibody-epr166732-western-blot.jpg)
Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 52, 50 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)](https://www.abcam.com/ps/products/178/ab178865/Images/ab178865-258147-anti-ppar-alpha-ppar-beta-delta-antibody-epr166732-western-blot.jpg)
Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : Mouse placenta lysate
Lane 6 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : Rat placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52, 50 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3,4 and 5: 2 seconds; Lane 6: 1 second; Lane 7: 30 seconds.
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![Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)](https://www.abcam.com/ps/products/178/ab178865/Images/ab178865-258144-anti-ppar-alpha-ppar-beta-delta-antibody-epr166732-western-blot.jpg)
Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1 : Mouse brain lysate at 10 µg
Lane 2 : Mouse heart lysate at 10 µg
Lane 3 : Mouse kidney lysate at 10 µg
Lane 4 : Mouse spleen lysate at 10 µg
Lane 5 : Rat brain lysate at 10 µg
Lane 6 : Rat heart lysate at 10 µg
Lane 7 : Rat kidney lysate at 10 µg
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52, 50 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3 and 4: 2 seconds; Lane 5,6,7,8,9,10 and 11: 1 second.
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![Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)](https://www.abcam.com/ps/products/178/ab178865/Images/ab178865-5-benefits-of-recombinant-antibodies.png)
Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab178865 has been referenced in 1 publication.
- Sharma S et al. Dichotomy of bisphenol A-induced expression of peroxisome proliferator-activated receptors in hepatic and testicular tissues in mice. Chemosphere 236:124264 (2019). PubMed: 31319301