Recombinant Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free (ab250082)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] - BSA and Azide free
See all PPAR alpha + PPAR beta/delta primary antibodies -
Description
Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250082 is the carrier-free version of ab178865.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16673(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 52, 50 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 52, 50 kDa). |
Images
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All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/10000 dilution
Lane 1 : Human PPAR alpha full length recombinant protein
Lane 2 : Human PPAR beta full length recombinant protein
Lane 3 : Human PPAR gamma fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52, 50 kDaThis data was developed using ab178865, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 10 seconds; Lane 2: 1 second; Lane 3: 3 minutes.
Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR beta full length recombinant protein contains aa1-441 with a His-Tag; Human PPAR gamma fragment recombinant protein contains aa1-200 with a His-Tag. These three recombinant proteins were made in-house.
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All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 52, 50 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis data was developed using ab178865, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : Mouse placenta lysate
Lane 6 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : Rat placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52, 50 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This data was developed using ab178865, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-5: 2 seconds; Lane 6: 1 second; Lane 7: 30 seconds.
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All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution
Lane 1 : Mouse brain lysate at 10 µg
Lane 2 : Mouse heart lysate at 10 µg
Lane 3 : Mouse kidney lysate at 10 µg
Lane 4 : Mouse spleen lysate at 10 µg
Lane 5 : Rat brain lysate at 10 µg
Lane 6 : Rat heart lysate at 10 µg
Lane 7 : Rat kidney lysate at 10 µg
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52, 50 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This data was developed using ab178865, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 2 seconds; Lanes 5-11: 1 second.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250082 has not yet been referenced specifically in any publications.