Anti-PPAR delta antibody (ab137724)
Key features and details
- Rabbit polyclonal to PPAR delta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PPAR delta antibody
See all PPAR delta primary antibodies -
Description
Rabbit polyclonal to PPAR delta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 129-378 of Human PPAR delta.
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Positive control
- Molt-4 and Mouse brown adipose tissue whole cell lysates; A549 Xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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| IHC-P |
1/100 - 1/1000.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Blocking condition: Incubate the sections in 3% H2O2 at room temperature for 30 minutes. Incubate the sections in 1.5% goat serum (dilute goat serum by 1xPBS) at room temperature for 30 minutes. |
| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Blocking condition: Incubate the sections in 3% H2O2 at room temperature for 30 minutes. Incubate the sections in 1.5% goat serum (dilute goat serum by 1xPBS) at room temperature for 30 minutes. |
Target
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Function
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
Tissue specificity
Ubiquitous with maximal levels in placenta and skeletal muscle. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 353106 Cow
- Entrez Gene: 481756 Dog
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Entrez Gene: 397297 Pig
- Entrez Gene: 25682 Rat
- Omim: 600409 Human
- SwissProt: Q0ZAQ8 Dog
see all -
Alternative names
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
Images
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Western blot - Anti-PPAR delta antibody (ab137724)Anti-PPAR delta antibody (ab137724) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Western blot - Anti-PPAR delta antibody (ab137724)Anti-PPAR delta antibody (ab137724) at 1/1000 dilution + Mouse brown adipose tissue whole cell lysate
at 50 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR delta antibody (ab137724)Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labelling PPAR delta with ab137724 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab137724 has been referenced in 6 publications.
- Shao M et al. Identification of the active compounds and drug targets of Chinese medicine in heart failure based on the PPARs-RXRa pathway. J Ethnopharmacol 257:112859 (2020). PubMed: 32294506
- Zhou X et al. Mature myelin maintenance requires Qki to coactivate PPARß-RXRa-mediated lipid metabolism. J Clin Invest 130:2220-2236 (2020). PubMed: 32202512
- Liang F et al. Melatonin Alleviates Neuronal Damage After Intracerebral Hemorrhage in Hyperglycemic Rats. Drug Des Devel Ther 14:2573-2584 (2020). PubMed: 32753840
- Maurya SK et al. Sarcolipin Signaling Promotes Mitochondrial Biogenesis and Oxidative Metabolism in Skeletal Muscle. Cell Rep 24:2919-2931 (2018). PubMed: 30208317
- Li XH et al. Neuroprotective effects of sodium hydrosulfide against ß-amyloid-induced neurotoxicity. Int J Mol Med 38:1152-60 (2016). WB ; Rat . PubMed: 27511125
- Maurya SK et al. Sarcolipin Is a Key Determinant of the Basal Metabolic Rate, and Its Overexpression Enhances Energy Expenditure and Resistance against Diet-induced Obesity. J Biol Chem 290:10840-9 (2015). PubMed: 25713078