Anti-PPAR delta antibody (ab23673)
Key features and details
- Rabbit polyclonal to PPAR delta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-PPAR delta antibody
See all PPAR delta primary antibodies -
Description
Rabbit polyclonal to PPAR delta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Chicken
-
Immunogen
Synthetic peptide corresponding to Human PPAR delta aa 1-100 (N terminal).
Database link: Q03181 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 4 µg/ml.
|
| WB | (6) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa. Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 4 µg/ml. |
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa. Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa. |
Target
-
Function
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
Tissue specificity
Ubiquitous with maximal levels in placenta and skeletal muscle. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 395479 Chicken
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Omim: 600409 Human
- SwissProt: Q03181 Human
- SwissProt: P35396 Mouse
- Unigene: 696032 Human
- Unigene: 328914 Mouse
-
Alternative names
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
Images
-
Western blot - Anti-PPAR delta antibody (ab23673)This image is courtesy of an anonymous AbreviewAnti-PPAR delta antibody (ab23673) at 1/1000 dilution + Rat brain tissue lysate at 100 µg
Secondary
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
-
Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab23673)Immunofluorescent staining of HT-29 cells (ab3952) (nuclear staining) using ab23673.
-
Western blot - Anti-PPAR delta antibody (ab23673)All lanes : Anti-PPAR delta antibody (ab23673)
Lane 1 : Human cerebral cortex (30ug)
Lane 2 : Murine liver (30ug)
Predicted band size: 50 kDa
ab23673 recognises PPAR delta at 50kDa from human samples. An additional smaller size of PPAR delta (~40kDa) is also detected in certain murine tissues.
Datasheets and documents
-
SDS download
-
Datasheet download
References (38)
ab23673 has been referenced in 38 publications.
- Wang YL et al. Laminar shear stress upregulates the expression of PPARs in vascular endothelial cells under high free fatty acid-induced stress. Exp Ther Med 21:438 (2021). PubMed: 33747175
- Xu L et al. Retinal homeostasis and metformin-induced protection are not affected by retina-specific Ppard knockout. Redox Biol 37:101700 (2020). PubMed: 32863184
- Shu J et al. Andrographolide protects against endothelial dysfunction and inflammatory response in rats with coronary heart disease by regulating PPAR and NF-?B signaling pathways. Ann Palliat Med 9:1965-1975 (2020). PubMed: 32692217
- Lee K et al. Gomisin N from Schisandra chinensis Ameliorates Lipid Accumulation and Induces a Brown Fat-Like Phenotype through AMP-Activated Protein Kinase in 3T3-L1 Adipocytes. Int J Mol Sci 21:N/A (2020). PubMed: 32245100
- Tang X et al. Activation of PPAR-ß/d Attenuates Brain Injury by Suppressing Inflammation and Apoptosis in a Collagenase-Induced Intracerebral Hemorrhage Mouse Model. Neurochem Res 45:837-850 (2020). PubMed: 31939088