Recombinant Anti-PPAR delta antibody [EPR18517] (ab178866)
![Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-266341-anti-ppar-delta-antibody-epr18517-western-blot.jpg "Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18517] to PPAR delta
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-PPAR delta antibody [EPR18517]
See all PPAR delta primary antibodies -
Description
Rabbit monoclonal [EPR18517] to PPAR delta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human PPAR delta full length recombinant protein; Jurkat, K562, HeLa, C6 and NIH/3T3 whole cell lysates; Mouse brain, heart and spleen lysates; Rat brain, heart and kidney lysates. IP: NIH/3T3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18517 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
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| IP |
1/20.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
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IP
1/20. |
Target
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Function
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
Tissue specificity
Ubiquitous with maximal levels in placenta and skeletal muscle. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Entrez Gene: 25682 Rat
- Omim: 600409 Human
- SwissProt: Q03181 Human
- SwissProt: P35396 Mouse
- Unigene: 696032 Human
- Unigene: 328914 Mouse
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Alternative names
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
Images
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Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)All lanes : Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1 : Human PPAR delta full length recombinant protein
Lane 2 : Human PPAR alpha full length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR delta full length recombinant protein contains aa1-441 with a His-Tag.
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![Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-266340-anti-ppar-delta-antibody-epr18517-western-blot.jpg)
Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)All lanes : Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 15 seconds.
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![Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-266339-anti-ppar-delta-antibody-epr18517-western-blot.jpg)
Western blot - Anti-PPAR delta antibody [EPR18517] (ab178866)All lanes : Anti-PPAR delta antibody [EPR18517] (ab178866) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1, 2, 3, 4, 5 and 6: 3 minutes; Lane 7 and 8: 10 seconds.
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![Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-266338-anti-ppar-delta-antibody-epr18517-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] (ab178866)PPAR delta was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab178866 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab178866 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate, 10µg (Input).
Lane 2: ab178866 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab178866 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-PPAR delta antibody [EPR18517] (ab178866)](https://www.abcam.com/ps/products/178/ab178866/Images/ab178866-5-benefits-of-recombinant-antibodies.png)
Anti-PPAR delta antibody [EPR18517] (ab178866)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab178866 has been referenced in 2 publications.
- Müller DIH et al. PPARd-mediated mitochondrial rewiring of osteoblasts determines bone mass. Sci Rep 10:8428 (2020). PubMed: 32439961
- Halstead AM et al. Bladder-cancer-associated mutations inRXRAactivate peroxisome proliferator-activated receptors to drive urothelial proliferation. Elife 6:N/A (2017). PubMed: 29143738