Recombinant Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)
![Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)](https://www.abcam.com/ps/products/223/ab223137/Images/ab223137-323034-anti-ppar-delta-antibody-epr18517-immunoprecipitation.jpg "Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18517] to PPAR delta - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-PPAR delta antibody [EPR18517] - BSA and Azide free
See all PPAR delta primary antibodies -
Description
Rabbit monoclonal [EPR18517] to PPAR delta - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human PPAR delta full length recombinant protein; Jurkat, K562, HeLa, C6 and NIH/3T3 whole cell lysates; Mouse brain, heart and spleen lysates; Rat brain, heart and kidney lysates. IP: NIH/3T3 whole cell lysate.
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General notes
ab223137 is the carrier-free version of ab178866.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18517 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
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IP
Use at an assay dependent concentration. |
Target
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Function
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
Tissue specificity
Ubiquitous with maximal levels in placenta and skeletal muscle. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Entrez Gene: 25682 Rat
- Omim: 600409 Human
- SwissProt: Q03181 Human
- SwissProt: P35396 Mouse
- Unigene: 696032 Human
- Unigene: 328914 Mouse
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Alternative names
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
Images
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Immunoprecipitation - Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)
PPAR delta was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab178866 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab178866 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate, 10µg (Input).
Lane 2: ab178866 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab178866 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178866).
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![Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)](https://www.abcam.com/ps/products/223/ab223137/Images/ab223137-2-benefits-of-recombinant-antibodies.png)
Anti-PPAR delta antibody [EPR18517] - BSA and Azide free (ab223137)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab223137 has been referenced in 1 publication.
- Wang L et al. Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR-? and UCP2. Mol Med Rep 18:789-798 (2018). PubMed: 29845235
