Recombinant Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)
![Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)](https://www.abcam.com/ps/products/234/ab234439/Images/ab234439-387020-anti-ppm1d430wip1s-antibody-epr2296039-western-blot-mcf7-jurkat-a549-f9-hct-116-raw-2647pc12-nih3t3-lysates.jpg "Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22960-39] to PPM1D/WIP1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PPM1D/WIP1 antibody [EPR22960-39]
See all PPM1D/WIP1 primary antibodies -
Description
Rabbit monoclonal [EPR22960-39] to PPM1D/WIP1 -
Host species
Rabbit -
Specificity
The antibody detects specifically PPM1D430.
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Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, Jurkat, A549, F9, HCT 116, RAW 264.7, PC-12 and NIH/3T3 whole cell lysate, Human testis and lung cancer tissue lysates, Mouse and Rat spleen tissue lysates. ICC/IF: MCF7, HCT-116 cells. Flow Cyt (intra): MCF7 cell.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22960-39 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
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| ICC/IF |
1/50.
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| WB |
1/1000.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
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ICC/IF
1/50. |
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WB
1/1000. |
Target
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Function
Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. -
Sequence similarities
Belongs to the PP2C family.
Contains 1 PP2C-like domain. - Information by UniProt
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Database links
- Entrez Gene: 8493 Human
- Entrez Gene: 53892 Mouse
- Omim: 605100 Human
- SwissProt: O15297 Human
- SwissProt: Q9QZ67 Mouse
- Unigene: 286073 Human
- Unigene: 45609 Mouse
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Alternative names
- EC 3.1.3.16 antibody
- p53 induced protein phosphatase 1 antibody
- p53-induced protein phosphatase 1 antibody
see all
Images
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Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)All lanes : Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lane 5 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1-4: 70 seconds; Lanes 5-8: 48 seconds.
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![Immunocytochemistry/ Immunofluorescence - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)](https://www.abcam.com/ps/products/234/ab234439/Images/ab234439-1-anti-ppm1d430wip1s-antibody-epr2296039-immunocytochemistry-mcf7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MCF7 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)](https://www.abcam.com/ps/products/234/ab234439/Images/ab234439-3-anti-ppm1d430wip1s-antibody-epr2296039-flow-cytometry-mcf7.jpg)
Flow Cytometry (Intracellular) - Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)](https://www.abcam.com/ps/products/234/ab234439/Images/ab234439-387022-anti-ppm1d430wip1s-antibody-epr2296039-western-blot-mouse-spleen-rat-spleen-lysates.jpg)
Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)All lanes : Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunocytochemistry/ Immunofluorescence - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)](https://www.abcam.com/ps/products/234/ab234439/Images/ab234439-2-anti-ppm1d430wip1s-antibody-epr2296039-immunocytochemistry-hct116.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT-116 (Human colorectal carcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HCT-116 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)](https://www.abcam.com/ps/products/234/ab234439/Images/ab234439-387021-anti-ppm1d430wip1s-antibody-epr2296039-western-blot-human-testis-human-lung-cancer-lysates.jpg)
Western blot - Anti-Secretory stress proteins (SSP) antibody [EPR22960-39] (ab234439)All lanes : Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human lung cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody (HRP))
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 3 minutes
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![Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)](https://www.abcam.com/ps/products/234/ab234439/Images/ab234439-7-benefits-of-recombinant-antibodies.png)
Anti-PPM1D/WIP1 antibody [EPR22960-39] (ab234439)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab234439 has not yet been referenced specifically in any publications.