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    products/primary-antibodies/ppp1ca--ppp1cb-antibody-ep1511y-bsa-and-azide-free-ab239844.pdf

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RecombinantRabMAb

Recombinant Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

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  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
  • Immunoprecipitation - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
  • Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
  • Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
  • Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt (Intra), IP, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
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HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
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Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (ab269823)

View more associated products

Overview

  • Product name

    Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free
    See all PPP1CA + PPP1CB primary antibodies
  • Description

    Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239844 is the carrier-free version of ab52619.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1511Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372)
    • Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab52619)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunohistochemistry kits

    • Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab239844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.

Target

  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus.
  • Database links

    • Entrez Gene: 5499 Human
    • Entrez Gene: 5500 Human
    • Entrez Gene: 19045 Mouse
    • Entrez Gene: 19046 Mouse
    • Entrez Gene: 24668 Rat
    • Entrez Gene: 25594 Rat
    • Omim: 176875 Human
    • Omim: 600590 Human
    • SwissProt: P62136 Human
    • SwissProt: P62140 Human
    • SwissProt: P62137 Mouse
    • SwissProt: P62141 Mouse
    • SwissProt: P62138 Rat
    • SwissProt: P62142 Rat
    see all
  • Alternative names

    • PP-1A antibody
    • PP-1B antibody
    • PP1A antibody
    • PP1alpha antibody
    • PP1B antibody
    • PP1beta antibody
    • PPP1A antibody
    • PPP1CA antibody
    • PPP1CB antibody
    • PPP1CD antibody
    • protein phosphatase 1 catalytic subunit alpha isozyme antibody
    • protein phosphatase 1 catalytic subunit beta isoform antibody
    • protein phosphatase 1 catalytic subunit delta isoform antibody
    • serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit antibody
    • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
    • Serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

    ab52619 staining PPP1CA + 1CB in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52619).

  • Immunoprecipitation - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
    Immunoprecipitation - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

    ab52619 (purified) at 1/30 immunoprecipitating PPP1CA + 1CB in Jurkat cell lysate. For western blotting, a HRP-conjugated Goat anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52619).

  • Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
    Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

    Overlay histogram showing HeLa cells stained with ab52619 (red line) at 1/150 dilution. The cells were fixed with 80% methanol. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line) 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52619). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

    ab52619 staining PP1CA + 1CB in Human cerebrum cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/50). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52619).

  • Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
    Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

    Overlay histogram showing HeLa cells stained with ab52619, unpurified  (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52619, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52619). 

     

     

  • Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)
    Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab239844? Please let us know so that we can cite the reference in this datasheet.

ab239844 has not yet been referenced specifically in any publications.

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