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    products/primary-antibodies/pr3-antibody-epr23253-35-ab270441.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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RecombinantRabMAb

Recombinant Anti-PR3 antibody [EPR23253-35] (ab270441)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
  • Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
  • Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
  • Anti-PR3 antibody [EPR23253-35] (ab270441)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23253-35] to PR3
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-PR3 antibody [EPR23253-35]
    See all PR3 primary antibodies
  • Description

    Rabbit monoclonal [EPR23253-35] to PR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt,IP or WB
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bone marrow, lung, spleen and placenta tissue; Mouse lung and spleen tissue. ICC/IF: HL-60, Mouse bone marrow cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23253-35
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes

Associated products

  • Alternative Versions

    • Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
1/100.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,IP or WB.

Target

  • Function

    Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
  • Sequence similarities

    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Target information above from: UniProt accession P24158 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5657 Human
    • Entrez Gene: 19152 Mouse
    • Omim: 177020 Human
    • SwissProt: P24158 Human
    • SwissProt: Q61096 Mouse
    • Unigene: 928 Human
    • Unigene: 2364 Mouse
    • Alternative names

      • ACPA antibody
      • AGP 7 antibody
      • AGP7 antibody
      • AGP7 serine proteinase antibody
      • Azurophil Granule Protein 7 antibody
      • C ANCA antibody
      • C ANCA antigen antibody
      • C-ANCA antigen antibody
      • CANCA antibody
      • EC 3.4.21.76 antibody
      • Leukocyte proteinase 3 antibody
      • MBN antibody
      • MBT antibody
      • MBT WEGENER AUTOANTIGEN antibody
      • Myeloblastin antibody
      • Neutrophil proteinase 4 antibody
      • NP 4 antibody
      • NP-4 antibody
      • NP4 antibody
      • P29 antibody
      • PR 3 antibody
      • PR-3 antibody
      • PR3 antibody
      • Proteinase 3 antibody
      • Proteinase3 antibody
      • PRTN 3 antibody
      • Prtn3 antibody
      • PRTN3_HUMAN antibody
      • Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody
      • Serine proteinase, neutrophil antibody
      • Wegener autoantigen antibody
      • Wegener granulomatosis autoantigen antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

      Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on human bone marrow (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)
      Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)

      ab270441 staining PR3 in HL-60 cells, with negative expression in K562 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab270441 at 5 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 µg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse spleen. The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)
      Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse bone marrow or EL4 cells labelling PR3 with ab270441 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in a subset of mouse bone marrow cells. Negative control: EL4 (PMID: 9211743). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse lung (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human placenta.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human spleen.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human lung (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-PR3 antibody [EPR23253-35] (ab270441)
      Anti-PR3 antibody [EPR23253-35] (ab270441)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab270441? Please let us know so that we can cite the reference in this datasheet.

    ab270441 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PR3 antibody [EPR23253-35]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Colon
    Blocking step
    Donkey Serum 10% + 3% BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Dylan Windell

    Verified customer

    Submitted May 05 2022

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