Recombinant Anti-PR3 antibody [EPR23253-35] (ab270441)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-1-anti-pr3-antibody-epr2325335-immunohistochemistry-human-bone-marrow.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23253-35] to PR3
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PR3 antibody [EPR23253-35]
See all PR3 primary antibodies -
Description
Rabbit monoclonal [EPR23253-35] to PR3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt,IP or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human bone marrow, lung, spleen and placenta tissue; Mouse lung and spleen tissue. ICC/IF: HL-60, Mouse bone marrow cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23253-35 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
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| IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
1/100. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Database links
- Entrez Gene: 5657 Human
- Entrez Gene: 19152 Mouse
- Omim: 177020 Human
- SwissProt: P24158 Human
- SwissProt: Q61096 Mouse
- Unigene: 928 Human
- Unigene: 2364 Mouse
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Alternative names
- ACPA antibody
- AGP 7 antibody
- AGP7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on human bone marrow (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-9-rabbit-monoclonal-epr2325335-to-pr3-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)ab270441 staining PR3 in HL-60 cells, with negative expression in K562 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab270441 at 5 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 µg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-6-anti-pr3-antibody-epr2325335-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse spleen. The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-7-anti-pr3-antibody-epr2325335-immunocytochemistry-mouse-bone-marrow-el4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse bone marrow or EL4 cells labelling PR3 with ab270441 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in a subset of mouse bone marrow cells. Negative control: EL4 (PMID: 9211743). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-5-anti-pr3-antibody-epr2325335-immunohistochemistry-mouse-lung.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse lung (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-4-anti-pr3-antibody-epr2325335-immunohistochemistry-human-placenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human placenta.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-3-anti-pr3-antibody-epr2325335-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human spleen.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-2-anti-pr3-antibody-epr2325335-immunohistochemistry-human-lung.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human lung (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-PR3 antibody [EPR23253-35] (ab270441)](https://www.abcam.com/ps/products/270/ab270441/Images/ab270441-8-benefits-of-recombinant-antibodies.png)
Anti-PR3 antibody [EPR23253-35] (ab270441)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab270441 has not yet been referenced specifically in any publications.