Recombinant Anti-PR3 antibody [EPR6277] (ab133613)
![Western blot - Anti-PR3 antibody [EPR6277] (ab133613)](https://www.abcam.com/ps/products/133/ab133613/Images/ab133613-147470-anti-pr3-antibody-epr6277-western-blot.jpg "Western blot - Anti-PR3 antibody [EPR6277] (ab133613)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6277] to PR3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PR3 antibody [EPR6277]
See all PR3 primary antibodies -
Description
Rabbit monoclonal [EPR6277] to PR3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HL60 and Human spleen lysates; Human kidney tissue
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6277 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 27 kDa).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 27 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.
Target
-
Function
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
-
Database links
- Entrez Gene: 5657 Human
- Omim: 177020 Human
- SwissProt: P24158 Human
- Unigene: 928 Human
-
Alternative names
- ACPA antibody
- AGP 7 antibody
- AGP7 antibody
see all
Images
-
Western blot - Anti-PR3 antibody [EPR6277] (ab133613)All lanes : Anti-PR3 antibody [EPR6277] (ab133613) at 1/1000 dilution
Lane 1 : HL60 lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR6277] (ab133613)](https://www.abcam.com/ps/products/133/ab133613/Images/ab133613-147469-anti-pr3-antibody-epr6277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR6277] (ab133613)Immunohistochemical analysis of PR3 in paraffin embedded Human kidney tissue labelled with ab133613 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![OI-RD Scanning - Anti-PR3 antibody [EPR6277] (ab133613)](https://www.abcam.com/ps/products/133/ab133613/Images/ab133613-147468-anti-pr3-antibody-epr6277.jpg)
OI-RD Scanning - Anti-PR3 antibody [EPR6277] (ab133613) -
![Anti-PR3 antibody [EPR6277] (ab133613)](https://www.abcam.com/ps/products/133/ab133613/Images/ab133613-4-benefits-of-recombinant-antibodies.png)
Anti-PR3 antibody [EPR6277] (ab133613)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab133613 has been referenced in 4 publications.
- Yang CW et al. Case Report: Proteinase 3 Antineutrophil Cytoplasmic Antibody-Associated Ulcerative Colitis Presenting as Recurrent Intestinal Pseudo-Obstruction in a Teenage Patient With in situ Proteinase 3 Immunohistochemical Staining. Front Pediatr 10:822491 (2022). PubMed: 35281249
- Seren S et al. Consequences of cathepsin C inactivation for membrane exposure of proteinase 3, the target antigen in autoimmune vasculitis. J Biol Chem 293:12415-12428 (2018). PubMed: 29925593
- Lepper MF et al. Proteomic Landscape of Patient-Derived CD4+ T Cells in Recent-Onset Type 1 Diabetes. J Proteome Res 17:618-634 (2018). PubMed: 29182335
- Ndika J et al. Epithelial proteome profiling suggests the essential role of interferon-inducible proteins in patients with allergic rhinitis. J Allergy Clin Immunol 140:1288-1298 (2017). PubMed: 28633877