Recombinant Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)
![Western blot - Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)](https://www.abcam.com/ps/products/248/ab248577/Images/ab248577-405989-anti-pr3-antibody-epr6277-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6277] to PR3 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PR3 antibody [EPR6277] - BSA and Azide free
See all PR3 primary antibodies -
Description
Rabbit monoclonal [EPR6277] to PR3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248577 is the carrier-free version of ab133613.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 5.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6277 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 27 kDa).
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| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Database links
- Entrez Gene: 5657 Human
- Omim: 177020 Human
- SwissProt: P24158 Human
- Unigene: 928 Human
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Alternative names
- ACPA antibody
- AGP 7 antibody
- AGP7 antibody
see all
Images
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Western blot - Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)All lanes : Anti-PR3 antibody [EPR6277] (ab133613) at 1/1000 dilution
Lane 1 : HL60 lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDaThis data was developed using ab133613, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)](https://www.abcam.com/ps/products/248/ab248577/Images/ab248577-2-anti-pr3-antibody-epr6277-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)This data was developed using ab133613, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of PR3 in paraffin embedded Human kidney tissue labelled with ab133613 at a 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![OI-RD Scanning - Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)](https://www.abcam.com/ps/products/248/ab248577/Images/ab248577-1-anti-pr3-antibody-epr6277-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)This data was developed using ab133613, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)](https://www.abcam.com/ps/products/248/ab248577/Images/ab248577-4-benefits-of-recombinant-antibodies.png)
Anti-PR3 antibody [EPR6277] - BSA and Azide free (ab248577)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248577 has not yet been referenced specifically in any publications.
