Anti-PRAME antibody (ab89097)
Key features and details
- Mouse polyclonal to PRAME
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PRAME antibody
See all PRAME primary antibodies -
Description
Mouse polyclonal to PRAME -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PRAME. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- HeLa whole cell lysate (ab150035), PRAME transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. |
Target
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Function
Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. -
Tissue specificity
Expressed in testis. Detected in samples of kidney, brain and skin. -
Sequence similarities
Belongs to the PRAME family.
Contains 4 LRR (leucine-rich) repeats. -
Cellular localization
Nucleus. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 23532 Human
- Omim: 606021 Human
- SwissProt: P78395 Human
- Unigene: 30743 Human
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Alternative names
- 4930534P07Rik antibody
- Cancer/testis antigen 130 antibody
- CT130 antibody
see all
Images
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Western blot - Anti-PRAME antibody (ab89097)Anti-PRAME antibody (ab89097) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PRAME antibody (ab89097)All lanes : Anti-PRAME antibody (ab89097) at 1 µg/ml
Lane 1 : PRAME transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab89097 has been referenced in 2 publications.
- Sivaccumar JP et al. Development of a New Highly Selective Monoclonal Antibody against Preferentially Expressed Antigen in Melanoma (PRAME) and Identification of the Target Epitope by Bio-Layer Interferometry. Int J Mol Sci 22:N/A (2021). PubMed: 33804612
- Nettersheim D et al. The cancer/testis-antigen PRAME supports the pluripotency network and represses somatic and germ cell differentiation programs in seminomas. Br J Cancer 115:454-64 (2016). PubMed: 27441500
