Recombinant Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)
![Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)](https://www.abcam.com/ps/products/243/ab243146/Images/ab243146-393196-AntiPRDM1-Blimp1-antibody-6D3-western-blot-nih3t3-raw2647-mouse.jpg "Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [6D3] to PRDM1/Blimp1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-PRDM1/Blimp1 antibody [6D3]
See all PRDM1/Blimp1 primary antibodies -
Description
Rat monoclonal [6D3] to PRDM1/Blimp1 -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- WB: NIH/3T3 and RAW 264.7 whole cell lysates. IHC-P: Mouse small intestine tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
6D3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.89 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 96 kDa).
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| IHC-P |
Use a concentration of 17.8 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
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WB
Use a concentration of 0.89 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 96 kDa). |
|
IHC-P
Use a concentration of 17.8 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
Post-translational
modificationsSumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 12142 Mouse
- SwissProt: Q60636 Mouse
- Unigene: 4800 Mouse
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Alternative names
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
Images
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Western blot - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)All lanes : Anti-PRDM1/Blimp1 antibody [6D3] (ab243146) at 0.89 µg/ml
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 96 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)](https://www.abcam.com/ps/products/243/ab243146/Images/ab243146-393195-AntiPRDM1-Blimp1-antibody-6D3-immunohistochemistry-small-intestine-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling PRDM1/Blimp1 with ab243146 at 17.8µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP). Nuclear staining on the lymph node and surface epithelial cells in the mouse small intestine (PMID: 21878906). Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)](https://www.abcam.com/ps/products/243/ab243146/Images/ab243146-3-benefits-of-recombinant-antibodies.png)
Anti-PRDM1/Blimp1 antibody [6D3] (ab243146)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab243146 has been referenced in 1 publication.
- Kallies A et al. Plasma cell ontogeny defined by quantitative changes in blimp-1 expression. J Exp Med 200:967-77 (2004). PubMed: 15492122