Anti-PRDM1/Blimp1 antibody (ab13700)
Key features and details
- Goat polyclonal to PRDM1/Blimp1
- Suitable for: ICC, ChIP, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PRDM1/Blimp1 antibody
See all PRDM1/Blimp1 primary antibodies -
Description
Goat polyclonal to PRDM1/Blimp1 -
Host species
Goat -
Tested applications
Suitable for: ICC, ChIP, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human PRDM1/Blimp1 aa 800 to the C-terminus (C terminal).
Database link: O75626 -
Positive control
- ICC: A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 10 µg/ml.
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ChIP |
Use at an assay dependent concentration. PubMed: 17264218
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC
Use a concentration of 10 µg/ml. |
ChIP
Use at an assay dependent concentration. PubMed: 17264218 |
Flow Cyt
Use at an assay dependent concentration. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
Post-translational
modificationsSumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 538384 Cow
- Entrez Gene: 481947 Dog
- Entrez Gene: 639 Human
- Entrez Gene: 12142 Mouse
- Entrez Gene: 100154284 Pig
- Entrez Gene: 309871 Rat
- Omim: 603423 Human
- SwissProt: O75626 Human
see all -
Alternative names
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
Images
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Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab13700 has been referenced in 10 publications.
- Qing K et al. Dysregulated MDR1 by PRDM1/Blimp1 Is Involved in the Doxorubicin Resistance of Non-Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma. Chemotherapy 67:12-23 (2022). PubMed: 34844236
- Li Q et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun 13:7677 (2022). PubMed: 36509766
- Okuzaki Y et al. PRDM14 and BLIMP1 control the development of chicken primordial germ cells. Dev Biol 455:32-41 (2019). PubMed: 31271752
- Chinnapaiyan S et al. TGF-ß1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells. Sci Rep 9:12552 (2019). PubMed: 31467373
- Prajapati RS et al. PRDM1 controls the sequential activation of neural, neural crest and sensory progenitor determinants. Development 146:N/A (2019). PubMed: 31806661
- Zhao H et al. GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing. Sci Rep 5:7913 (2015). IF . PubMed: 25604641
- Beermann ML et al. Prdm1 (Blimp-1) and the expression of fast and slow myosin heavy chain isoforms during avian myogenesis in vitro. PLoS One 5:e9951 (2010). PubMed: 20376350
- Wang X et al. RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1. Mol Cell Biol 29:3832-44 (2009). PubMed: 19433448
- Yan J et al. BLIMP1 regulates cell growth through repression of p53 transcription. Proc Natl Acad Sci U S A 104:1841-6 (2007). ChIP ; Human . PubMed: 17264218
- Parekh S et al. BCL6 programs lymphoma cells for survival and differentiation through distinct biochemical mechanisms. Blood 110:2067-74 (2007). PubMed: 17545502