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RecombinantRabMAb

Recombinant Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

Submit a review Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
  • Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16655] to PRDM1/Blimp1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PRDM1/Blimp1 antibody [EPR16655]
    See all PRDM1/Blimp1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16655] to PRDM1/Blimp1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human diffuse large B cell lymphoma, tonsil and cerebral cortex tissues. IF: RAMOS cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/250.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
1/250.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or WB.

Target

  • Function

    Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).

  • Sequence similarities

    Belongs to the class V-like SAM-binding methyltransferase superfamily.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SET domain.

  • Post-translational
    modifications

    Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
    Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.

  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • B Lymphocyte Induced Maturation Protein 1 antibody
      • Beta interferon gene positive regulatory domain I binding factor antibody
      • Beta-interferon gene positive regulatory domain I-binding factor antibody
      • BLIMP-1 antibody
      • BLIMP1 antibody
      • Positive Regulatory Domain I Binding Factor 1 antibody
      • Positive regulatory domain I-binding factor 1 antibody
      • PR Domain Containing 1 antibody
      • PR domain containing 1 with ZNF domain antibody
      • PR domain containing 1 with ZNF domain isoform 2 antibody
      • PR domain containing protein 1 antibody
      • PR domain zinc finger protein 1 antibody
      • PR domain-containing protein 1 antibody
      • PRDI BF1 antibody
      • PRDI binding factor 1 antibody
      • PRDI-BF1 antibody
      • PRDI-binding factor 1 antibody
      • PRDM 1 antibody
      • Prdm1 antibody
      • PRDM1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

      Immunohistochemical analysis of paraffin-embedded Human diffuse large B cell lymphoma tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human diffuse large B cell lymphoma tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

      Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
      Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAMOS (Human Burkitt's lymphoma cell line) cells, labeling PRDM1/Blimp1 with ab198287 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear and weakly cytoplasm staining on RAMOS cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:
      1. ab198287 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
      Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

    References (3)

    Publishing research using ab198287? Please let us know so that we can cite the reference in this datasheet.

    ab198287 has been referenced in 3 publications.

    • Li Q  et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun 13:7677 (2022). PubMed: 36509766
    • Wang WF  et al. HSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells. Nat Commun 8:363 (2017). PubMed: 28842558
    • Xia Y  et al. Loss of PRDM1/BLIMP-1 function contributes to poor prognosis of activated B-cell-like diffuse large B-cell lymphoma. Leukemia N/A:N/A (2016). PubMed: 27568520

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