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RecombinantRabMAb

Recombinant Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
  • Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16655] to PRDM1/Blimp1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free
    See all PRDM1/Blimp1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16655] to PRDM1/Blimp1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240344 is the carrier-free version of ab198287.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).

  • Sequence similarities

    Belongs to the class V-like SAM-binding methyltransferase superfamily.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SET domain.

  • Post-translational
    modifications

    Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
    Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.

  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • B Lymphocyte Induced Maturation Protein 1 antibody
      • Beta interferon gene positive regulatory domain I binding factor antibody
      • Beta-interferon gene positive regulatory domain I-binding factor antibody
      • BLIMP-1 antibody
      • BLIMP1 antibody
      • Positive Regulatory Domain I Binding Factor 1 antibody
      • Positive regulatory domain I-binding factor 1 antibody
      • PR Domain Containing 1 antibody
      • PR domain containing 1 with ZNF domain antibody
      • PR domain containing 1 with ZNF domain isoform 2 antibody
      • PR domain containing protein 1 antibody
      • PR domain zinc finger protein 1 antibody
      • PR domain-containing protein 1 antibody
      • PRDI BF1 antibody
      • PRDI binding factor 1 antibody
      • PRDI-BF1 antibody
      • PRDI-binding factor 1 antibody
      • PRDM 1 antibody
      • Prdm1 antibody
      • PRDM1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)

      Immunohistochemical analysis of paraffin-embedded Human diffuse large B cell lymphoma tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human diffuse large B cell lymphoma tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198287).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198287).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)

      Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198287).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
      Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAMOS (Human Burkitt's lymphoma cell line) cells, labeling PRDM1/Blimp1 with ab198287 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear and weakly cytoplasm staining on RAMOS cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:
      1. ab198287 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198287).

    • Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)
      Anti-PRDM1/Blimp1 antibody [EPR16655] - BSA and Azide free (ab240344)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240344? Please let us know so that we can cite the reference in this datasheet.

    ab240344 has not yet been referenced specifically in any publications.

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