Recombinant Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
![Western blot - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)](https://www.abcam.com/ps/products/307/ab307644/Images/ab307644-499757-anti-prdm1blimp1-antibody-epr25163-80-western-blot-sr-human-pleural-effusion-lymphoblast-whole-cell-lysate20-ug.jpg "Western blot - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25163-80] to PRDM1/Blimp1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-PRDM1/Blimp1 antibody [EPR25163-80]
See all PRDM1/Blimp1 primary antibodies -
Description
Rabbit monoclonal [EPR25163-80] to PRDM1/Blimp1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SR lysates IHC-P: Human tonsil tissues. IP: SR cell
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25163-80 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 100 kDa.
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 100 kDa. |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/30. |
Target
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Function
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
Post-translational
modificationsSumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 639 Human
- Omim: 603423 Human
- SwissProt: O75626 Human
- Unigene: 436023 Human
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Alternative names
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
Images
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Western blot - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)All lanes : Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644) at 1/1000 dilution
Lane 1 : SR (human pleural effusion lymphoblast) whole cell lysate
Lane 2 : Raji (human burkitts lymphoma b lymphocyte) whole cell lysate
Lane 3 : NAMALWA (human burkitts lymphoma b lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Raji, NAMALWA (PMID: 28842558).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: 180 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)](https://www.abcam.com/ps/products/307/ab307644/Images/ab307644-2-anti-prdm1blimp1-antibody-epr25163-80-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PRDM1/Blimp1 with ab307644 at 1/500 (0.924 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Nuclear staining on human tonsil (PMID: 24438193). The section was incubated with ab307644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)](https://www.abcam.com/ps/products/307/ab307644/Images/ab307644-3-anti-prdm1blimp1-antibody-epr25163-80-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRDM1/Blimp1 with ab307644 at 1/500 (0.924 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on human liver. The section was incubated with ab307644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunoprecipitation - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)](https://www.abcam.com/ps/products/307/ab307644/Images/ab307644-4-anti-prdm1blimp1-antibody-epr25163-80-immunoprecipitation-sr.jpg)
Immunoprecipitation - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)PRDM1/Blimp1 was immunoprecipitated from 0.35 mg SR (human pleural effusion lymphoblast) whole cell lysate 10 µg with ab307644 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab307644 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: SR (human pleural effusion lymphoblast) whole cell lysate 10 µg
Lane 2: ab307644 IP in SR whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307644 in SR whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
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![Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)](https://www.abcam.com/ps/products/307/ab307644/Images/ab307644-1-benefitsofrecombinantantibodies.jpg)
Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab307644 has not yet been referenced specifically in any publications.