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    products/primary-antibodies/prdm1blimp1-antibody-epr25163-80-ab307644.pdf

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Immunology Immunoglobulins Class Switching
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RecombinantRabMAb

Recombinant Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
  • Immunoprecipitation - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
  • Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25163-80] to PRDM1/Blimp1
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

HRP HRP

Overview

  • Product name

    Anti-PRDM1/Blimp1 antibody [EPR25163-80]
    See all PRDM1/Blimp1 primary antibodies
  • Description

    Rabbit monoclonal [EPR25163-80] to PRDM1/Blimp1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt (Intra) or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SR lysates IHC-P: Human tonsil tissues. IP: SR cell
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR25163-80
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Class Switching
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Arginine Methylation

Associated products

  • Alternative Versions

    • Anti-PRDM1/Blimp1 antibody [EPR25163-80] - BSA and Azide free (ab307645)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab307644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 100 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
Notes
WB
1/1000. Predicted molecular weight: 100 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
Application notes
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

Target

  • Function

    Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
  • Sequence similarities

    Belongs to the class V-like SAM-binding methyltransferase superfamily.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • Post-translational
    modifications

    Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
    Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession O75626 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 639 Human
    • Omim: 603423 Human
    • SwissProt: O75626 Human
    • Unigene: 436023 Human
    • Alternative names

      • B Lymphocyte Induced Maturation Protein 1 antibody
      • Beta interferon gene positive regulatory domain I binding factor antibody
      • Beta-interferon gene positive regulatory domain I-binding factor antibody
      • BLIMP-1 antibody
      • BLIMP1 antibody
      • Positive Regulatory Domain I Binding Factor 1 antibody
      • Positive regulatory domain I-binding factor 1 antibody
      • PR Domain Containing 1 antibody
      • PR domain containing 1 with ZNF domain antibody
      • PR domain containing 1 with ZNF domain isoform 2 antibody
      • PR domain containing protein 1 antibody
      • PR domain zinc finger protein 1 antibody
      • PR domain-containing protein 1 antibody
      • PRDI BF1 antibody
      • PRDI binding factor 1 antibody
      • PRDI-BF1 antibody
      • PRDI-binding factor 1 antibody
      • PRDM 1 antibody
      • Prdm1 antibody
      • PRDM1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
      Western blot - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
      All lanes : Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644) at 1/1000 dilution

      Lane 1 : SR (human pleural effusion lymphoblast) whole cell lysate
      Lane 2 : Raji (human burkitts lymphoma b lymphocyte) whole cell lysate
      Lane 3 : NAMALWA (human burkitts lymphoma b lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 100 kDa
      Observed band size: 100 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: Raji, NAMALWA (PMID: 28842558).

       

      Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

      Exposure time: 180 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PRDM1/Blimp1 with ab307644 at 1/500 (0.924 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Nuclear staining on human tonsil (PMID: 24438193). The section was incubated with ab307644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRDM1/Blimp1 with ab307644 at 1/500 (0.924 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Negative control: No staining on human liver. The section was incubated with ab307644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunoprecipitation - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
      Immunoprecipitation - Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)

      PRDM1/Blimp1 was immunoprecipitated from 0.35 mg SR (human pleural effusion lymphoblast) whole cell lysate 10 µg with ab307644 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab307644 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: SR (human pleural effusion lymphoblast) whole cell lysate 10 µg

      Lane 2: ab307644 IP in SR whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307644 in SR whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 50 seconds

      Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

    • Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)
      Anti-PRDM1/Blimp1 antibody [EPR25163-80] (ab307644)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab307644? Please let us know so that we can cite the reference in this datasheet.

    ab307644 has not yet been referenced specifically in any publications.

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