Anti-PRDM1/Blimp1 antibody [ROS195G] - BSA and Azide free (ab252785)
![Western blot - Anti-PRDM1/Blimp1 antibody [ROS195G] - BSA and Azide free (ab252785)](https://www.abcam.com/ps/products/252/ab252785/Images/ab252785-480264-241568AP5976583PRDM1Test6102.jpg "Western blot - Anti-PRDM1/Blimp1 antibody [ROS195G] - BSA and Azide free (ab252785)")
Key features and details
- Mouse monoclonal [ROS195G] to PRDM1/Blimp1 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PRDM1/Blimp1 antibody [ROS195G] - BSA and Azide free
See all PRDM1/Blimp1 primary antibodies -
Description
Mouse monoclonal [ROS195G] to PRDM1/Blimp1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Positive control
- ICC/IF: A431 cells WB: Human kidney tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate.
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General notes
ab252785 is the carrier-free version of ab241568
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
ROS195G -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 91 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 91 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
Sequence similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
Post-translational
modificationsSumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 639 Human
- Entrez Gene: 12142 Mouse
- Entrez Gene: 309871 Rat
- Omim: 603423 Human
- SwissProt: O75626 Human
- SwissProt: Q60636 Mouse
- Unigene: 436023 Human
- Unigene: 4800 Mouse
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Alternative names
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
Images
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Western blot - Anti-PRDM1/Blimp1 antibody [ROS195G] - BSA and Azide free (ab252785)All lanes : Anti-PRDM1/Blimp1 antibody [ROS195G] (ab241568) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Raji whole cell lysate
Lane 5 : PT-6967 whole cell lysate
Lane 6 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 91 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab241568). Lanes 1 - 6: Merged signal (red and green). Green - ab241568 observed at 85 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa. ab241568 was shown to react with PRDM1/Blimp1 in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween ®) before incubation with ab241568 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye ® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye ® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [ROS195G] - BSA and Azide free (ab252785)](https://www.abcam.com/ps/products/252/ab252785/Images/ab252785-1-anti-prdm1-blimp1-ros195g-antibody-immunocytochemistry-a431-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [ROS195G] - BSA and Azide free (ab252785)This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab241568).
ab241568 staining PRDM1/Blimp1 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab241568 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab252785 has not yet been referenced specifically in any publications.
