Recombinant Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
![Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)](https://www.abcam.com/ps/products/76/ab76083/Images/ab76083-297755-anti-presenilin-1-ps-1-antibody-ep2000y-western-blot.jpg "Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2000Y] to Presenilin 1/PS-1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Presenilin 1/PS-1 antibody [EP2000Y]
See all Presenilin 1/PS-1 primary antibodies -
Description
Rabbit monoclonal [EP2000Y] to Presenilin 1/PS-1 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Presenilin 1/PS-1 aa 300-400. The exact sequence is proprietary.
Database link: P49768 -
Positive control
- WB: HeLa, Mouse brain, Rat brain cell, Human fetal brain lysate. IHC-P: Human liver.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2000Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/5000. Predicted molecular weight: 53 kDa.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 53 kDa. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis. -
Tissue specificity
Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes. -
Involvement in disease
Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty. -
Sequence similarities
Belongs to the peptidase A22A family. -
Domain
The PAL motif is required for normal active site conformation. -
Post-translational
modificationsHeterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils. - Information by UniProt
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Database links
- Entrez Gene: 5663 Human
- Entrez Gene: 19164 Mouse
- Entrez Gene: 29192 Rat
- Omim: 104311 Human
- SwissProt: P49768 Human
- SwissProt: P49769 Mouse
- SwissProt: P97887 Rat
- Unigene: 3260 Human
see all -
Alternative names
- AD3 antibody
- Ad3h antibody
- FAD antibody
see all
Images
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Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Presenilin 1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HEK293 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab76083 observed at 18 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab76083 was shown to recognize Presenilin 1 in wild-type HAP1 cells as signal was lost at the expected MW in Presenilin 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Presenilin 1 knockout samples were subjected to SDS-PAGE. Ab76083 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)](https://www.abcam.com/ps/products/76/ab76083/Images/ab76083-333680-anti-presenilin-1-ps-1-antibody-ep2000y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling Presenilin 1/PS-1 with Purified ab76083 at 1:100 dilution (1.31 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)](https://www.abcam.com/ps/products/76/ab76083/Images/ab76083-333646-anti-presenilin-1-ps-1-antibody-ep2000y-western-blot.jpg)
Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)All lanes : Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083) at 1/1000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?The 18 KD band is the C-terminal fragment after proteolytic process reported by PMID 9173929. But we are not sure how to define the extra band around 75 KD.
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![Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)](https://www.abcam.com/ps/products/76/ab76083/Images/ab76083-333635-anti-presenilin-1-ps-1-antibody-ep2000y-western-blot.jpg)
Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083) at 1/1000 dilution (Purified) + Human fetal brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?The 18 KD band is the C-terminal fragment after proteolytic process reported by PMID 9173929.
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![Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)](https://www.abcam.com/ps/products/76/ab76083/Images/ab76083-10-benefits-of-recombinant-antibodies.png)
Anti-Presenilin 1/PS-1 antibody [EP2000Y] (ab76083)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab76083 has been referenced in 37 publications.
- Xin X et al. miR-141-3p regulates saturated fatty acid-induced cardiomyocyte apoptosis through Notch1/PTEN/AKT pathway via targeting PSEN1. Environ Toxicol 37:741-753 (2022). PubMed: 34897970
- Wei W & Zhang Y PSEN1 is associated with colon cancer development via potential influences on PD-L1 nuclear translocation and tumor-immune interactions. Front Immunol 13:927474 (2022). PubMed: 36059511
- Long QH et al. Suan-Zao-Ren Decoction ameliorates synaptic plasticity through inhibition of the Aß deposition and JAK2/STAT3 signaling pathway in AD model of APP/PS1 transgenic mice. Chin Med 16:14 (2021). PubMed: 33478552
- Li YZ et al. Combined treatment with valproic acid and estrogen has neuroprotective effects in ovariectomized mice with Alzheimer's disease. Neural Regen Res 16:2078-2085 (2021). PubMed: 33642397
- Lam SM et al. A multi-omics investigation of the composition and function of extracellular vesicles along the temporal trajectory of COVID-19. Nat Metab 3:909-922 (2021). PubMed: 34158670