Anti-Prickle 2 antibody (ab211419)
Key features and details
- Rabbit polyclonal to Prickle 2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Prickle 2 antibody -
Description
Rabbit polyclonal to Prickle 2 -
Host species
Rabbit -
Specificity
ab211419 is predicted not to cross-react with other Prickle protein family memebers. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Prickle 2 (internal sequence). The exact sequence is proprietary. The synthetic peptide corresponds to 15 amino acids. NCBI Accession No. NP_942559.1.
Database link: Q7Z3G6 -
Positive control
- Mouse A20 cell lysate, Human testis tissue and rat brain cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 20 µg/ml.
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|
IHC-P |
Use a concentration of 5 µg/ml.
Perform heat-mediated antigen retrieval. |
|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 96 kDa.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat-mediated antigen retrieval. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 96 kDa. |
Target
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Tissue specificity
Expressed in brain, eye and testis. Additionally in fetal brain, adult cartilage, pancreatic islet, gastric cancer and uterus tumors. -
Involvement in disease
Epilepsy, progressive myoclonic 5 -
Sequence similarities
Belongs to the prickle / espinas / testin family.
Contains 3 LIM zinc-binding domains.
Contains 1 PET domain. -
Cellular localization
Nucleus membrane. - Information by UniProt
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Database links
- Entrez Gene: 166336 Human
- Entrez Gene: 243548 Mouse
- Entrez Gene: 312563 Rat
- Omim: 608501 Human
- SwissProt: Q7Z3G6 Human
- SwissProt: Q80Y24 Mouse
- Unigene: 148105 Human
- Unigene: 248820 Mouse
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Alternative names
- EPM5 antibody
- PRIC2 antibody
- PRIC2_HUMAN antibody
see all
Images
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Anti-Prickle 2 antibody (ab211419) at 1 µg/ml + mouse A20 cell lysate
Predicted band size: 96 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, labeling Prickle 2 using ab211419 at 5 µg/ml.
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Immunofluorescence analysis of rat brain cells, labeling Prickle 2 using ab211419 at a 20 µg/mL.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab211419 has been referenced in 1 publication.
- Huang C et al. Circular RNA hsa-circ-000881 suppresses the progression of lung adenocarcinoma in vitro via a miR-665/PRICKLE2 axis. Ann Transl Med 9:498 (2021). PubMed: 33850895