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RecombinantRabMAb

Recombinant Anti-PRMT1 antibody [EPR18344] (ab190892)

Submit a review Submit a question References (4)
Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Immunoprecipitation - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)
  • Anti-PRMT1 antibody [EPR18344] (ab190892)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18344] to PRMT1
  • Suitable for: IP, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-PRMT1 antibody [EPR18344]
    See all PRMT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18344] to PRMT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, NIH/3T3, A549, C6, RAW 264.7 and PC-12 cell lysates. IHC-P: Human colon, Human breast cancer, rat colon and mouse liver tissue. ICC/IF: HeLa cells. ChIP: Chromatin prepared from MCF-7+ß-estraiol 30min, and MCF-7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/80.
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/2000.
Notes
IP
1/80.
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/2000.

Target

  • Function

    Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.

  • Tissue specificity

    Widely expressed.

  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.

  • Cellular localization

    Nucleus. Cytoplasm > cytosol.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ANM 1 antibody
    • ANM1 antibody
    • ANM1_HUMAN antibody
    • HCP 1 antibody
    • HCP1 antibody
    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody
    • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody
    • Histone-arginine N-methyltransferase PRMT1 antibody
    • HMT 2 antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
    • HMT1 hnRNP methyltransferase antibody
    • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
    • HMT1 hnRNP methyltransferase like 2 antibody
    • HMT2 antibody
    • HRMT1 L2 antibody
    • HRMT1L 2 antibody
    • HRMT1L2 antibody
    • Human mRNA for suppressor for yeast mutant antibody
    • Human mRNA for suppressor for yeast mutant complete cds antibody
    • Interferon receptor 1 bound protein 4 antibody
    • Interferon receptor 1 bound protein4 antibody
    • Interferon receptor 1-bound protein 4 antibody
    • Interferon receptor 1bound protein 4 antibody
    • IR1 B4 antibody
    • IR1B 4 antibody
    • IR1B4 antibody
    • Mrmt 1 antibody
    • Mrmt1 antibody
    • PRMT 1 antibody
    • PRMT1 antibody
    • Protein arginine methyltransferase 1 antibody
    • Protein arginine N methyltransferase 1 antibody
    • Protein arginine N methyltransferase1 antibody
    • Protein arginine N-methyltransferase 1 antibody
    • R1B4 antibody
    • S. cerevisiae like 2 antibody
    see all

Images

  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
    All lanes : Anti-PRMT1 antibody [EPR18344] (ab190892) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human colon tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PRMT1 with ab190892 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab190892 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/500 dilution.
    -ve control 2:  anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by  Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution.

  • Immunoprecipitation - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Immunoprecipitation - Anti-PRMT1 antibody [EPR18344] (ab190892)

    PRMT1 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab190892 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab190892 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293 whole cell lysate 10ug (Input).

    Lane 2: ab190892 IP in HEK-293 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190892 in HEK293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds.

     

  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Anti-PRMT1 antibody [EPR18344] (ab190892) at 1/1000 dilution + A549 (Human lung carcinoma cell line) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining on Human breast cancer tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Western blot - Anti-PRMT1 antibody [EPR18344] (ab190892)
    All lanes : Anti-PRMT1 antibody [EPR18344] (ab190892) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) cell lysate
    Lane 2 : RAW264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining and weak cytoplasmic staining on rat colon tissue is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT1 antibody [EPR18344] (ab190892)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling PRMT1 with ab190892 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    It has been shown that in living cells PRMT1 shuttles between the nucleus and the cytoplasm depending on the methylation status of substrate proteins. Genes to Cells (2009) 14, 309–317.

  • Anti-PRMT1 antibody [EPR18344] (ab190892)
    Anti-PRMT1 antibody [EPR18344] (ab190892)

References (4)

Publishing research using ab190892? Please let us know so that we can cite the reference in this datasheet.

ab190892 has been referenced in 4 publications.

  • Chen X  et al. Curcumin attenuates endothelial cell fibrosis through inhibiting endothelial-interstitial transformation. Clin Exp Pharmacol Physiol 47:1182-1192 (2020). PubMed: 32020664
  • Kumar A  et al. Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription. Cell Rep 32:108172 (2020). PubMed: 32997990
  • Yang RH  et al. Interleukin enhancement binding factor 3 inhibits cardiac hypertrophy by targeting asymmetric dimethylarginine-nitric oxide. Nitric Oxide 93:44-52 (2019). PubMed: 31536826
  • Sen S  et al. PRMT1 Plays a Critical Role in Th17 Differentiation by Regulating Reciprocal Recruitment of STAT3 and STAT5. J Immunol 201:440-450 (2018). PubMed: 29794014

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