Recombinant Anti-PRMT3 antibody [EPR13279] (ab191562)
![Western blot - Anti-PRMT3 antibody [EPR13279] (ab191562)](https://www.abcam.com/ps/products/191/ab191562/Images/ab191562-229120-anti-prmt3-antibody-epr13279-western-blot.jpg "Western blot - Anti-PRMT3 antibody [EPR13279] (ab191562)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13279] to PRMT3
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PRMT3 antibody [EPR13279]
See all PRMT3 primary antibodies -
Description
Rabbit monoclonal [EPR13279] to PRMT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, 293 and MCF7 cell lysates IHC: Human cervix carcinoma tissue ICC/IF: MCF7 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13279 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/50.
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| IP |
1/40.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/50. |
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IP
1/40. |
Target
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Function
Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins. -
Sequence similarities
Belongs to the protein arginine N-methyltransferase family.
Contains 1 C2H2-type zinc finger. -
Domain
The zinc-finger is responsible for substrate specificity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10196 Human
- Omim: 603190 Human
- SwissProt: O60678 Human
- Unigene: 152337 Human
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Alternative names
- 2010005E20Rik antibody
- 2410018A17Rik antibody
- AL033309 antibody
see all
Images
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Western blot - Anti-PRMT3 antibody [EPR13279] (ab191562)All lanes : Anti-PRMT3 antibody [EPR13279] (ab191562) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] (ab191562)](https://www.abcam.com/ps/products/191/ab191562/Images/ab191562-229119-anti-prmt3-antibody-epr13279-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRMT3 antibody [EPR13279] (ab191562)Immunohistochemical analysis of paraffin-embedded, human cervix carcinoma tissue labeling PRMT3 with ab191562 at a 1/100 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] (ab191562)](https://www.abcam.com/ps/products/191/ab191562/Images/ab191562-229118-anti-prmt3-antibody-epr13279-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PRMT3 antibody [EPR13279] (ab191562)Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling PRMT3 with ab191562 at a 1/50 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor® 488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PRMT3 at a 1/50 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.
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![Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] (ab191562)](https://www.abcam.com/ps/products/191/ab191562/Images/ab191562-229117-anti-prmt3-antibody-epr13279-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PRMT3 antibody [EPR13279] (ab191562)Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling PRMT3 using ab191562 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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![Anti-PRMT3 antibody [EPR13279] (ab191562)](https://www.abcam.com/ps/products/191/ab191562/Images/ab191562-5-benefits-of-recombinant-antibodies.png)
Anti-PRMT3 antibody [EPR13279] (ab191562)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab191562 has been referenced in 7 publications.
- Lei Y et al. Protein arginine methyltransferase 3 promotes glycolysis and hepatocellular carcinoma growth by enhancing arginine methylation of lactate dehydrogenase A. Clin Transl Med 12:e686 (2022). PubMed: 35090076
- Liao Y et al. PRMT3 drives glioblastoma progression by enhancing HIF1A and glycolytic metabolism. Cell Death Dis 13:943 (2022). PubMed: 36351894
- Liu L et al. Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair. Sci Adv 8:eadd8928 (2022). PubMed: 36475791
- Verma M et al. PRMT3 interacts with ALDH1A1 and regulates gene-expression by inhibiting retinoic acid signaling. Commun Biol 4:109 (2021). PubMed: 33495566
- Yin S et al. PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis. Nat Commun 12:3444 (2021). PubMed: 34103528
- Zhang X et al. PRMT3 promotes tumorigenesis by methylating and stabilizing HIF1α in colorectal cancer. Cell Death Dis 12:1066 (2021). PubMed: 34753906
- Kumar A et al. Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription. Cell Rep 32:108172 (2020). PubMed: 32997990