Recombinant Anti-PRMT6 antibody [EPR24230-93] (ab271091)
![Western blot - Anti-PRMT6 antibody [EPR24230-93] (ab271091)](https://www.abcam.com/ps/products/271/ab271091/Images/ab271091-477795-anti-prmt6-antibody-epr2423093-western-blot-adenocarcinoma.jpg "Western blot - Anti-PRMT6 antibody [EPR24230-93] (ab271091)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24230-93] to PRMT6
- Suitable for: WB, IP, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PRMT6 antibody [EPR24230-93]
See all PRMT6 primary antibodies -
Description
Rabbit monoclonal [EPR24230-93] to PRMT6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: ChIP or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Hela transfected with PRMT6 siRNA 1, Hela transfected with PRMT6 siRNA 2, MCF7, SH-SY5Y, LNCaP, Human testis, Human kidney lysates. ICC/IF: MCF7 cells. Flow Cyt - intra: MCF7 cells. IP: SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24230-93 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 41 kDa.
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| IP |
1/30.
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| ICC/IF |
1/50.
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| Flow Cyt (Intra) |
1/50.
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| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 41 kDa. |
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IP
1/30. |
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ICC/IF
1/50. |
|
Flow Cyt (Intra)
1/50. |
Target
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Function
Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). -
Tissue specificity
Highly expressed in kidney and testis. -
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.
Contains 1 SAM-dependent MTase PRMT-type domain. -
Post-translational
modificationsAutomethylation enhances its stability and antiretroviral activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55170 Human
- Omim: 608274 Human
- SwissProt: Q96LA8 Human
- Unigene: 26006 Human
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Alternative names
- ANM6_HUMAN antibody
- Chromobox protein homolog 7 antibody
- FLJ10559 antibody
see all
Images
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Western blot - Anti-PRMT6 antibody [EPR24230-93] (ab271091)All lanes : Anti-PRMT6 antibody [EPR24230-93] (ab271091) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : Hela transfected with PRMT6 siRNA 1 whole cell lysate
Lane 3 : Hela transfected with PRMT6 siRNA 2 whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 6 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW/expression profile is consistent with what has been described in the literature (PMID: 29262320).
Exposure time: 3 minutes
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![Immunoprecipitation - Anti-PRMT6 antibody [EPR24230-93] (ab271091)](https://www.abcam.com/ps/products/271/ab271091/Images/ab271091-5-anti-prmt6-antibody-epr2423093-immunoprecipitation-shsy5y.jpg)
Immunoprecipitation - Anti-PRMT6 antibody [EPR24230-93] (ab271091)PRMT6 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab271091 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271091 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab271091 IP in SH-SY5Y whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab271091 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-PRMT6 antibody [EPR24230-93] (ab271091)](https://www.abcam.com/ps/products/271/ab271091/Images/ab271091-3-anti-prmt6-antibody-epr2423093-immunocytochemistry-mcf7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PRMT6 antibody [EPR24230-93] (ab271091)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 cells labelling PRMT6 with ab271091 at 1/50 (11.56 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive nuclear staining in MCF7 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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![Flow Cytometry (Intracellular) - Anti-PRMT6 antibody [EPR24230-93] (ab271091)](https://www.abcam.com/ps/products/271/ab271091/Images/ab271091-4-anti-prmt6-antibody-epr2423093-flow-cytometry-mcf7.jpg)
Flow Cytometry (Intracellular) - Anti-PRMT6 antibody [EPR24230-93] (ab271091)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling PRMT6 with ab271091 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-PRMT6 antibody [EPR24230-93] (ab271091)](https://www.abcam.com/ps/products/271/ab271091/Images/ab271091-477796-anti-prmt6-antibody-epr2423093-western-blot-lysate.jpg)
Western blot - Anti-PRMT6 antibody [EPR24230-93] (ab271091)All lanes : Anti-PRMT6 antibody [EPR24230-93] (ab271091) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW/expression profile is consistent with what has been described in the literature (PMID: 29262320).
-
![Anti-PRMT6 antibody [EPR24230-93] (ab271091)](https://www.abcam.com/ps/products/271/ab271091/Images/ab271091-6-Benefits-of-Recombinant-antibodies.jpg)
Anti-PRMT6 antibody [EPR24230-93] (ab271091)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab271091 has not yet been referenced specifically in any publications.