Recombinant Anti-PRMT7 antibody [EPR13495] (ab181214)
![Western blot - Anti-PRMT7 antibody [EPR13495] (ab181214)](https://www.abcam.com/ps/products/181/ab181214/Images/ab181214-215096-anti-prmt7-antibody-epr13495-western-blot.png "Western blot - Anti-PRMT7 antibody [EPR13495] (ab181214)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13495] to PRMT7
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PRMT7 antibody [EPR13495]
See all PRMT7 primary antibodies -
Description
Rabbit monoclonal [EPR13495] to PRMT7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, HeLa, 293 and MCF7 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13495 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. -
Involvement in disease
Defects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP). -
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
Contains 2 SAM-dependent MTase PRMT-type domains. -
Cellular localization
Cytoplasm, cytosol. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 54496 Human
- Omim: 610087 Human
- SwissProt: Q9NVM4 Human
- Unigene: 640229 Human
- Unigene: 653193 Human
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Alternative names
- [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody
- ANM7_HUMAN antibody
- FLJ10640 antibody
see all
Images
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Western blot - Anti-PRMT7 antibody [EPR13495] (ab181214)All lanes : Anti-PRMT7 antibody [EPR13495] (ab181214) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 78 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PRMT7 antibody [EPR13495] (ab181214)](https://www.abcam.com/ps/products/181/ab181214/Images/ab181214-215097-anti-prmt7-antibody-epr13495-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PRMT7 antibody [EPR13495] (ab181214)Immunofluorescent analysis of HeLa cells (-20℃ acetone-fixed) labeling PRMT7 with ab181214 at 1/250 dilution (red). Goat anti rabbit IgG (Alexa Fluor® 555) was used as a secondary antibody, at a dilution of 1/200. Counter stain: Dapi (blue).
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![Anti-PRMT7 antibody [EPR13495] (ab181214)](https://www.abcam.com/ps/products/181/ab181214/Images/ab181214-3-benefits-of-recombinant-antibodies.png)
Anti-PRMT7 antibody [EPR13495] (ab181214)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab181214 has been referenced in 4 publications.
- Shen T et al. An enhancer variant at 16q22.1 predisposes to hepatocellular carcinoma via regulating PRMT7 expression. Nat Commun 13:1232 (2022). PubMed: 35264579
- Jeong A et al. PRMT7 Inhibitor SGC8158 Enhances Doxorubicin-Induced DNA Damage and Its Cytotoxicity. Int J Mol Sci 23:N/A (2022). PubMed: 36293180
- Zhang W et al. The role of protein arginine methyltransferase 7 in human developmentally arrested embryos cultured in vitro. Acta Biochim Biophys Sin (Shanghai) 53:925-932 (2021). PubMed: 34041522
- Kumar A et al. Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription. Cell Rep 32:108172 (2020). PubMed: 32997990